摘要
采用L9(34)正交设计,以小檗碱的含量为指标,优选降糖胶囊中黄连的提取方法。用HPLC 法测定小檗碱的含量,流动相为磷酸二氢钾硫代硫酸钠水乙腈(3-4g∶1-7g∶140ml∶60ml)。结果表明:最佳提取条件为80 % 乙醇回流3 次,第1 次6 倍量回流2h,第2 次5 倍量回流1-5h,第3 次4 倍量回流1-5h。
By determining berberine with HPLC method,the optimum extraction conditions of rhizoma coptidis in Jiangtang capsule were established.The mobile phase of HPLC was KH 2PO 4 Na 2S 2O 3 H 2O CH 3CN(3 4g∶1 7g∶140ml∶60ml).The best extraction process was as follows:reflux with 80% alcohol for three times,for the first time:6 times alcohol of crude drug for 2hr;the second:5times for 1 5hr and the last:4 times for 1 5hr.
出处
《中国实验方剂学杂志》
CAS
2000年第1期12-14,共3页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
降糖胶囊
黄连
小檗碱
正交设计
中药
Jiangtang capsule
rhizoma coptidis
Berberine
orthogonal design method