高效液相色谱法测定农田人参中9种人参皂苷单体含量

Content Determination of 9 Ginsenoside Monomers in Farmland Ginseng by HPLC

为评价农田人参质量,建立同时测定农田人参中9种人参皂苷单体含量的方法。采用反相高效液相色谱法(reversed phase-high performance liquid chromatography,RP-HPLC)对农田人参与伐林人参中9种人参皂苷单体含量进行比较分析,色谱条件:色谱柱(4.6mm×150mm,5μm),乙腈(A)-水(B)为流动相,梯度洗脱[0min(18%A)→24min(22%A)→26min(26%A)→30min(32%A)→50min(33.5%A)→55min(38%A)],流速为1.0mL/min,检测波长203 nm,柱温35℃。结果表明:农田人参含有与伐林人参相同种类的9种人参皂苷Rg 1、Re、Rf、Rg2、Rb1、Rc、Rb2、Rb3、Rd;6年生农田人参9种皂苷含量均高于6年生伐林人参,但除Rg1含量差异显著外(P<0.05),其他8种皂苷含量均不显著(P>0.05);4年生农田人参除Rg1、Rf显著高于4年生伐林人参(P<0.05)外,其他7种皂苷含量与4年生伐林人参差异均不显著(P>0.05)。农田人参中Rgl、Rb1的含量、Rgl和Re含量之和、Rgl和Re含量之和均超过中国药典、欧洲药典与美国药典的要求。

In order to evaluate the quality of farmland ginseng,a simple and accurate HPLC method for determining 9 ginsenoside monomers in farmland ginseng was established.The contents of 9 ginsenoside monomers were analyzed comparatively in farmland ginseng and wild ginseng by RP-HPLC.The chromatographic separation was achieved on a C18column（SHIMADZU,4.6 mm × 150 mm,5μm） using a mobile phase made up of acetonitrile（A） and water（B） at a flow rate of 1.0 mL/min in the following gradient mode： 0 min（18% A）→24 min（22% A）→26 min（26% A）→30 min（32% A）→50 min（33.5% A）→55 min（38% A）.The detection wavelength and column temperature were set as 203 nm and 35 ℃,respectively.The results indicated that nine kinds of ginsenoside monomers（Rg1,Re,Rf,Rg2,Rb1,Rc,Rb2,Rb3 and Rd） in both farmland ginseng and wild ginseng were detected.The contents of 9 ginsenoside monomers in six-year-old farmland ginseng were higher than six-year-old wild ginseng.However,no significant difference（P 0.05） was observed except for the content of ginsenoside monomer Rg1（P 0.05）.Similarly,no significant difference in the contents of ginsenoside monomers between four-year-old farmland ginseng and four-year-old wild ginseng（P 0.05） was observed except the contents of ginsenoside monomer Rg1 and Rf（P 0.05）.The contents of Rg1 and Rb1,total content of Rg1 and Re,and total content of Rb1 and Re in farmland ginseng met the quality standards of Chinese Pharmacopoeia,European Pharmacopoeia and American Pharmacopoeia.Therefore,farmland ginseng offers a potential alternative to wild ginseng for medicinal applications.