当归内酯拮抗环孢菌素A、氢化可的松及抗肿瘤药物的免疫抑制作用

Antagonistic effect of Angelica Sinests(Oliv. ) Diels Lactone(ASDL) on the immunosuppressive response induced by Cyclosporin A, Hydrocortisone and antitumor agents

目的：观察当归内酯（ＡＳＤＬ）对免疫抑制剂及抗肿瘤药物引起的免疫抑制作用的影响。方法：应用免疫抑制剂及抗肿瘤药物建立免疫功能低下的模型，使用不同剂量的ＡＳＤＬ进行体内外拮抗实验。结果：ＡＳＤＬ在６２．５，１２５．０，２５０．０ｕｇ／ｍｌ时，可部分或完全拮抗环孢菌素Ａ、丝裂霉素Ｃ、５－氟尿嘧啶和阿糖胞苷对小鼠混合淋巴细胞培养反应（ＭＬＲ）的轻度抑制作用（抑制５０％以下），部分拮抗氢化可的松对ＭＬＲ的重度抑制（抑制５０％以上）作用。ＡＳＤＬ１０及２０ｍｇ／（ｋｇ·ｄ）×７，腹腔注射可拮抗环磷酰胺（ＣＹ，８０ｍｇ／ｋｇ，ｓ．ｃ）对小鼠脾脏Ｔ淋巴细胞增殖、ＮＫ细胞活性、ＩＬ－２产生的免疫抑制作用。结论：当归内酯可通过不同途径拮抗化学因素引发的免疫抑制作用。

Objective: To study antagonistic effect of Angelica Sinests(Oliv. ) Diels Lactone (ASDL) on the immunosuppressive responseinduced by Cyclosporin A, Hydrocortisone and antitumor agents. Methods: Immunodeficient models were used in the experiments. Immunosup-pressive response was induced by immunosuppressive and antitumor agents. In the different concentration of ASDL, antagonistic experiments wereobserved in vivo and vitro. Results: ASDL (62.5, 125.0, 250.0 ug/ml) could completely antagothe the mild inhibition (less than 50% ) ofmurine mixed lymphocyte reaction (MLR) caused by Cyclosporin, Mitomycin C, 5-Fluorouracil, Cytarabine and partly antagonize the seriousinhibition (more than 50% ) induced by Hydrcortisone in vitro. ASDL [10 or 20 MG/(kg. d)× 7, i. p] could antagonize inhibition of immunologi-cal function caused by cyclophosphamide (CY,80 mg/kg, s. c), which could promote the declined T lymphocyte proliferation, restore the reduced NK Cell cytotoxicity and productipn of IL-2. Conclusion: ASDL could antagonize immunosuppressive response induced by chemical agents.