山羊乳牙牙髓干细胞构建组织工程骨的异位成骨研究

Dental Pulp Stem Cells from Goat Deciduous Teeth in Ectopic Osteogenesis

目的:评价山羊乳牙牙髓干细胞在体外的成骨分化能力,及复合多孔磷酸钙骨水泥在山羊肌袋模型内的异位成骨能力。方法:采用改良组织块法培养山羊乳牙牙髓干细胞,并且在成骨诱导条件下培养。经成骨条件培养的第3代细胞与多孔磷酸钙骨水泥复合后,植入山羊背部左侧肌袋;将多孔磷酸钙骨水泥空白支架植入右侧肌袋作为对照组。分别在植入后4、8周取材,进行组织学观察。结果:复合山羊乳牙牙髓干细胞的支架材料在4周后有类骨质形成;植入8周后,类骨质形成更多;未复合细胞的支架材料在组织学检查中未见有类骨质形成。结论:山羊乳牙牙髓干细胞在体外具有分化为成骨细胞的能力;多孔磷酸钙复合山羊乳牙牙髓干细胞在山羊体内具有异位成骨能力。

Objective： The aim of this study was to examine the osteogenic differentiation potential of dental pulp stem cells from goat deciduous teeth（SGDs） in vitro.Methods： SGDs were grown in modified tissue culture medium and cells from the third passage were used for experimentation.SGDs with porous calcium phosphate cement（pCPC） were transplanted into the goat left dorsal muscle pouch,and the pCPC without cells were transplanted into the right dorsal muscle pouch as negative controls.The transplants were harvested at the end of 4,8 weeks and prepared for histological evaluation.Results： Examination showed obvious bone formation at 4 weeks in SGDs-pCPC group and the amount of bone formation increased at 8 weeks after transplantation.Bone formation was not detected in only pCPC group.Conclusion： SGDs express the potential to differentiate into osteoblasts in vitro;SGDs combined with scaffold such as pCPC have the ability of ectopic osteogenesis.