微透析与现代分析技术在线联用的研究进展

Progress in the Online Coupling of in vivo Microdialysis with Modern Analysis Systems

目的:微透析技术是一种新型的实时连续活体采样技术。微透析技术与灵敏度高,选择性好的现代分析技术的在线联用真正实现了内源性物质和体内药物的实时测定,可有效缩短取样时间,提高了样品的稳定性,并能节省时间和节约成本,提高了时间分辨率以准确观察该快速变化,是体内药物分析的重大突破。进行微透析在线联用分析时需要综合考虑探针回收率,灌流速度、待测物相对分子量等对探针回收率的影响,以及分析需要的样品体积,待测物的浓度范围,方法的灵敏度和定量限,分析速度等相关影响因素,各因素之间相互联系。文章对目前应用较广的微透析与HPLC,CE,微芯片电泳和生物传感器等分析技术在线联用的研究进展进行综述,重点讨论不同进样接口,色谱柱及检测器对该联用技术的影响。

Microdialysis（MD） is a sampling technique that can be employed to monitor biological events continuously.When it is coupled to an sensitive and selective analytical system online,microdialysis can provide near realtime information on the time-dependent concentration changes of analytes in the extracellular space.Online systems for the analysis of microdialysis samples enable fast,selective and sensitive analysis while preserving the temporal information.It can improve the stability of the samples and save the time and cost.When developing an online assay based on microdialysis sampling,several parameters must be taken into consideration.These parameters include： the recovery of the probe,volume of sample available for analysis,concentration range of analyte in the sample,sensitivity and limit of detection of the analytical method scheme and the speed of analytical method.Analytical methods employed for online analysis include liquid chromatography（LC）,capillary（CE） and microchip electrophoresis and flow-through biosensor devices.Factors that affect the online systems include the interfaces,the column and the detectors was discussed.