次氯酸钠用于植物培养基灭菌方法初探

The Study on Sodium Hypochlorite for Sterilization of Plant Culture Medium

为了解决培养基高压蒸汽灭菌时间长、能源消耗多等问题,对植物组织培养基的灭菌方法进行了改进,在培养基的配制过程中添加不同浓度次氯酸钠进行灭菌。结果表明,在培养基配制过程中,琼脂加热溶解后,趁热在培养基中添加10mg/L次氯酸钠,未发现污染。以‘神马’菊花为外植体,在培养基配制过程中加入终浓度20mg/L的次氯酸钠时,植株可以正常生长,而且,生长状况好于高压灭菌组;次氯酸钠浓度高于50mg/L时,生长状况差于高压灭菌组,而且会产生伤害。在本试验条件下,利用次氯酸钠代替高压蒸汽灭菌具有一定的可行性;该灭菌方法具有缩短灭菌时间,节约能源等优点。

AbstSterilization methods in the culture medium of plants were improved so that the time of pressure steam sterilization and energy consumption could be shortened. Different concentration sodium hypochlorite was added to sterilization during the process of making culture medium. The results showed that during the media preparation process, the dissolved agar was heated, 10 mg/L sodium bypochlorite was supplemented into the hot medium, polluted culture medium was not found. With ＇God Ma＇ chrysanthemum explants in culture medium during the preparation of the final concentration 20 mg/L of sodium hypochlorite, all the plants grew normally, furthermore, their growth conditions were better than that of the group with the high-pressure sterilization. When sodium hypochlorite concentrations were above 50 mg/L, the growth was not only poorer than that of the control, but also did harm to them. In these conditions, the proper concentration of sodium hypochlorite replacing high-pressure steam sterilization was effective. This method could shorten the time and energy consumption.