基于EST-SSR研究芝麻地方种质不同大小繁殖群体间多态性

Study on Polymorphism of Different Reproduction Populations of Sesame (Sesamum indicum L.) Landrace Based on EST-SSR

从1份芝麻地方种质的200株总样本中随机抽取20、25、30、35、40、45、50、55个单株组成不同大小的群体,基于15对EST-SSR标记从DNA水平上分析不同群体间多态性特点。结果表明,群体样本大小至少为30～35株时可代表该种质的遗传特性,田间实际采用不小于60～70株作为芝麻地方种质的繁殖更新群体将能较好避免基因漂变,此研究结果将为确定基因库芝麻地方种质资源繁殖更新适宜的样本大小提供理论依据。

Different bulks with 20,25,30,35,40,45,50,55 plants were formed from an accession of 200 plants of a sesame landrace by randomly sampling.Genetic diversity of different bulks was analyzed by 15 pairs of EST-SSR markers at the DNA level.The results showed that the effective population size should be greater than 30-35 plants in order to represent the genetic characteristics of this germplasm,while it should be not less than 60-70 plants as appropriate population for reproduction of sesame landrace,to avoid gene drift and maintain the stability of genetic characteristics.