HPLC比较醇提重楼及其颗粒中重楼皂苷Ⅰ，Ⅱ的含量

Comparative on Content of Paridis Saponins Ⅰ,Ⅱ between Ethanol-Extraction and Granule of Rhizoma Paridis by HPLC

目的:建立一种测定重楼皂苷Ⅰ,Ⅱ的含量测定方法,用于比较重楼乙醇提物与重楼配方颗粒中皂苷的含量。方法:采用C18色谱柱,以乙腈-水(42∶58)为流动相,检测波长210 nm,流速1 mL.min-1,柱温室温。结果:在0.043 5～0.870 0g.L-1,重楼皂苷Ⅰ的峰面积与浓度有良好的线性,r=0.999 1;在0.038～0.760 0 g.L-1,重楼皂苷Ⅱ的峰面积与浓度有良好的线性,r=0.999 7,重楼皂苷Ⅰ,Ⅱ的平均回收率为99.90%,100.2%,RSD分别为1.7%,1.7%。醇提物与配方颗粒中皂苷Ⅰ,Ⅱ的总含量分别为34.7%,3.1%。结论:该法操作简单,出峰时间短,两峰分离度好,测得的醇提物中皂苷Ⅰ,Ⅱ含量明显高于重楼配方颗粒。

Objective： To establish a determination method of the paridis saponins Ⅰ and Ⅱ for content comparison of ethanol-extraction and granule of paridis μm） was used. Acetonitrile-water （42： 58） was used Method： Kromasil as a mobile phase. Cxscolumn （4.6 mm x Flow rate was 1 mL·min the 250 -1 Detection wavelength was at 210 nm, column temperature was in room temperature. Result： The Paridis saponins Ⅰ concentration and peak area was showed good linear relationship between 0. 0435-0.87μ L-1 （ r = 0. 999 1 ）. Paridis saponins Ⅱ concentration and peak area was showed good linear relationship between 0. 038-0. 76 g ·L-1 （r =0. 999 7） . recovery of Paridis saponins Ⅰ and saponins Ⅱ was 99.90% and 100.2% separately. RSD is 1.6% and 1.34% , the content of paridis saponin Ⅰ and Ⅱ in ethanol-extraction and granule is 34.7% and 3.1% respectively. Conclusion： This method is simple with short ratention time and well separation, the content of saponin Ⅰand Ⅱ in ethanol-extraction were significantly higher than that of granule.