摘要
目的:观察黄芩素对4种肝毒性物质诱导的人正常肝细胞株L-02细胞损伤的拮抗作用。方法:培养的L-02细胞分别给予10 mmol.L-1对乙酰氨基酚(AP),50μmol.L-1山岗橐吾碱(clivorine,CLI),2μmol.L-1川楝素(toosendanin,TSN)和100 mmol.L-1乙醇(EtOH)诱导肝细胞毒性。黄芩素1,10,25,50,100μmol.L-1分别与细胞预孵15 min后,加入肝毒性物质,48 h后MTT法检测细胞存活率。结果:与对照组相比,4种肝毒性物质均能显著降低肝细胞存活率(P<0.001)。经不同浓度黄芩素处理后,各浓度黄芩素均能显著提高AP,TSN,EtOH肝细胞损伤模型组的细胞存活率(P<0.01,P<0.001);10,25,50,100μmol.L-1黄芩素能显著提高CLI肝细胞损伤模型组的细胞存活率(P<0.05,P<0.001)。结论:黄芩素具有拮抗对乙酰氨基酚、山岗橐吾碱、川楝素和乙醇这些肝毒性物质诱导的肝细胞毒性的作用,且具有一定的浓度依赖性。
Objective: The present study is designed to observe the protective effects of baicalein against hepatotoxins induced cytotoxicity on the human normal liver L-02 cells. Method: L-02 cells were seeded and pretreated with or without baicalein (1, 10, 25, 50, 100μmol·L-1) for 15 min, and then cells were further treated with or without various hepatotoxins, including acetaminophen (AP, 10 mmol· L-l) , clivorine (CLI, 50μmol·L-1), toosendanin (TSN, 2 μmol·L-1) and ethanol (EtOH, 100μmol·L-1) for 48 h. The survival ceils were determined by MTT assay. Result: Four hepatotoxins could significantly reduce the cell viability (P 〈 0. 001) compared with the control group. After treatments, baicalein could increase the cell viability which was decreased by AP, TSN and EtOH (P 〈 0.01, P 〈 0. 001 ). Moreover, baicalein could significantly increase the cell viability which was decreased by CLI (P 〈 0.05, P 〈 0. 001 ). Conclusion: Baicalein can prevent the cytotoxicity induced by those hepatotoxins in human normal liver L-02 cells.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第6期145-148,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
上海市科技启明星计划(跟踪)(10QH1402200)
国家自然科学基金项目(青年基金30801544)
关键词
黄芩素
肝毒性
肝细胞
baicalein
hepatotoxicity
hepatocyte