繁殖群体量及隔离对蚕豆种质遗传完整性的影响

Effect of Sample Sizes and Isolation Methods on Genetic Integrity of Faba Bean(Vicia faba L. )Germplasm

为研究繁殖群体量和隔离方式对常异花授粉作物蚕豆种质繁殖更新的影响,以9份蚕豆地方种质为对象,以国家库保存的原种为对照群体,采用AFLP分子标记方法,对比了50株和20株群体量及开放无隔离群体和网棚隔离群体更新后代的遗传完整性差异。结果表明:与对照群体相比,50株和20株群体的多态性位点数、多态位点百分率、每位点有效等位基因数、香农指数、遗传多样性指数均出现不同程度下降,但下降幅度20株大于50株群体;遗传相似性和UPGMA聚类分析表明50株群体与对照群体的遗传相似性高于20株群体;网棚隔离可降低群体间串粉与花粉污染,但其遗传完整性却较开放无隔离群体低。

The genetic integrity of nine faba bean landraces and their regeneration groups were studied to inves- tigate the effects of sample sizes and isolation methods on the regeneration of faba bean （ Vicia faba L. ） using AFLP markers. Seeds stored in the national genebank were used as control. The results showed that： compared with those of the control group, the number of polymorphic loci, percentage of polymorphic loci, effective number of alleles per locus, Shannon index,and genetic diversity index decreased in 50 and 20 plants groups,but the decline was greater in 20 plants group. Genetic similarity analysis and UPGMA cluster results showed that the genetic similarity between control and 50 plants group was higher than that between control and 20 plants group. Cages isolation can reduce pollen contamination, but their genetic integrity was lower than that of the open pollination groups.