气调包装对白灵菇褐变及生物有效性的影响

Effect of Modified Atmosphere on Browning and Bioavailability of in vitro Digests of Pleurotus nebrodensis

在4℃、相对湿度95%的环境中,采用不同厚度PVC膜、PE膜(0.03mm,0.05mm)和微孔膜(0.015mm)保鲜白灵菇,贮藏期28d,定期检测O2和CO2体积分数、色差、多酚氧化酶(polyphenol oxidase,PPO)、多糖生物利用度等生理生化指标,研究被动自发气调对白灵菇褐变和多糖生物有效性的影响。结果表明:在贮藏的第7天,0.05mm薄膜包装内可形成稳定的低氧高二氧化碳的气体环境,并能维持21d,气体体积分数分别为O20.3%～2.1%,CO26%～10.3%,推迟了PPO酶活性高峰的到来,抑制白灵菇PPO酶活性升高;在贮藏前期能较好的维持多糖生物有效性;而不同包装对白灵菇颜色影响不显著。因此,厚度为0.05mm的薄膜自发气调包装能较好地抑制白灵菇褐变,减缓白灵菇营养成分的损失,延长白灵菇贮藏期。

In order to gain insight into the effect of positive modified amaosphere on the browning and polysaccharide bioavailability of Pleurotus nebrodensis, such physiological and biochemical indexes as oxygen and carbon dioxide concentrations, color difference, polyphenol oxidase （PPO） activity and polysaccharide bioavailability were measured on Pleurotus nebrodensis modified atmospheric packaged with PVC or PE films of different thickness （0.03 or 0.05 mm）or microporous film （0.015 mm） at regular time intervals during 28 days of storage at 4 ℃ and 95% relative humidity. A low-oxygen, high-carbon dioxide stable environment composed of 0.3 %-2.1% 02 and 6%-10.3 % CO2 was formed by 0.05 mm thick film on storage day 7 and could keep for 21 days. The environment could delay the occurrence of the PPO activity peak, inhibit the increase of PPO activity, and maintain polysac- charide bioavailability during the early part of the storage period. Different packages had no significant difference in their effect on the color of Pleurotus nebrodensis. Consequently, modified atmosphere packaging with 0.05 mm thick film could inhibit the browning of Pleurotus nebrodensis well, reduce the loss of nutrients and extend the storage life of Pleurotus nebrodensis.