H22荷瘤小鼠早期邪毒证与气虚证对甲状腺Tg等基因转录及激素水平的影响

Effects of Pathogenic Toxin and Qi-deficiency Syndrome on Tg and Other Genes' Transcription and Hormone Level in H22 Tumor-bearing Mice

目的:观察H22肝癌小鼠早期邪毒证和气虚证对甲状腺Tg等11个基因转录及激素水平影响。方法:采用标准化诊法技术筛选出邪毒证、气虚证H22肿瘤小鼠,RTqPCR检测激素生成相关Tshr等11个基因转录水平,同时ELISA法检测血清甲状腺激素T3和T4水平。结果:(1)甲状腺激素水平在肿瘤发生后下降,且邪毒证甚于气虚证;(2)Tg等11个基因,转录水平变化总体趋势与激素水平类似。结论:肿瘤发生后,小鼠甲状腺机能受到抑制,其中邪毒壅盛证尤甚。

Objective： To observe the effect of pathogenic toxin and qi-deficiency syndrome on Tg transcription and hormone level in H22 tumor-bearing mice.Methods： The standardized diagnosis were used in H22 tumor-bearing mice to select the pathogenic toxin syndrome and qi-deficiency syndrome,and RTqPCR was used to detect 11 gene transcription level involved in hormone biosynthesis,and ELISA was used to detect T3 and T4 levels in serum.Results：（1） Thyroid hormone in serum decreased after tumorigenesis in two groups,especially in pathogenic toxin syndrome.（2） Eleven genes in thyroid showed the same trend as hormone level in serum.Conclusion： After tumorigenesis,the thyroid function is inhibited especially in pathogenic toxin syndrome.