三套管法大鼠原位小肠移植动物模型的改进

Modified three-cuff technique for orthotopic intestinal transplantation in rats

目的 对三套管法大鼠原位小肠移植动物模型进行改进，提高原位小肠移植的成功率。方法取106只SD大鼠作为供、受体进行同种同基因原位无造瘘全小肠移植，动静脉吻合采用改良三套管法，其中受体肠系膜上静脉套管采用手术显微镜下套管。供体小肠近端与受体近端空肠端端吻合，远端与受体升结肠端端吻合。手术后存活超过3d即认为手术成功。结果手术成功率为90．6％（48／53），供体小肠热缺血时间为0，冷缺血时间为（30±2．48）rain，手术成功后7d存活率为97．9％（47／48）。结论在三套管技术的基础上，采用手术显微镜辅助下肠系膜上静脉套管，大大提高了该动物模型的成功率，缩短了手术技术的成熟时间。

Objective To modify the technique of whole small bowel transplantation in rats to improve the survival. Methods One hundred and six SD rats served as donors and recipients to establish a homogeneous and orthotopic model of small bowel transplantation without fistula. Anastomosis of aorta and vein was done with three-cuff technique, the vena mesenterica superior of recipient was done under a surgical microscope. End-to-end anastomosis was performed between donor proximal small intestine and receptor jejunum, and also between the remote and receptor ascending colon. Surviving more than 3 days after surgery was defined as the operation was successful. Results The operation was successfully carried out in 48 cases with the survival rate of 90. 6% （48/53）. The average warm ischemic time was 0 minute, and the average cold ischemictime was（30＋-2.48）min. Thesurvivalrate（ 〉7 d） was97.9%（47~48）. Conclusions On the basis of three-cuff technique, the survival of SBT was greatly improved. When the vena mesenterica superior of recipient was done under a surgical microscope, the surgical training time was shortened.