丹参酮对脊髓缺血再灌注损伤细胞因子TNF-α、IL-1β、IL-1Ra的影响

Effects of Tanshinone on the Changes of TNF-α,IL-1β and IL-1Ra in Rabbit Model with Spinal Cord Ischemia Reperfusion Injury

目的:通过观察细胞因子TNF-α、IL-1β、IL-1Ra指标,研究丹参酮对脊髓缺血再灌注损伤免疫途径的干预作用。方法:将新西兰家兔12只,随机分为单纯缺血再灌注组(模型组)、丹参酮干预组(丹参酮组)。采用Zivin法改进复制模型,于造模前1h治疗;丹参酮组,腹腔注射丹参酮。模型组,不做治疗处理。各组家兔分别于治疗前、缺血30min再灌注后0.5h、1h、4h、8h、12h分别采家兔股静脉血。检测:血清TNF-α、IL-1β、IL-1Ra指标。结果:模型组,家兔血清TNF-α、IL-1β、IL-1Ra各时点均升高,与正常时比较差异有统计学意义(P<0.05)。丹参酮组与模型组比较TNF-α、IL-1β降低(P<0.05);IL-1Ra升高(P<0.05)。结论:家兔脊髓缺血再灌注损伤后,可导致家兔血清TNF-α、IL-1β、IL-1Ra升高。丹参酮对脊髓缺血再灌注损伤免疫途径的干预作用是通过降低TNF-α、IL-1β,升高IL-1Ra,从而对脊髓神经功能有一定的保护作用。丹参酮治疗存在时间窗效应。

Objective：To observe the effect of Tanshinone on spinal cord ischemia reperfusion injury in rabbit model by the changes of TNF-α, IL-1β and IL-1Ra. Methods：Twelve New Zealand rabbits were randomly divided into tow groups： the untreated group and the Tanshinone group. The models of spinal cord ischemia reperfusion injury were built by the Zivin criterion. The rabbits in the untreated group were treated with nothing; the rabbits in the Tanshinone group were treated with intraperitoneal injection. Blood was collected at different time points： before ischemia injury; lh; 4h; 8h~ and 12h after reperfusion by rabbit femoral venous. TNF-α, IL-1β, and IL-1Ra levels were examined as marker of inflammation. Results： The levels of TNF-α, IL-1β and IL- 1Ra in the untreated group increased significantly after ischemia （P〈0.05）. The levels of TNF-α, IL-1β were declined in the Tanshinone group （P 〈0.05） and the level of IL-1Ra were increased at 4h after reperfusion （P〈0.05）, compared to that in the untreated group. Conclusions： The levels of TNF-α, IL-1β and IL-1Ra were increased in the models of spinal cord ischemia reperfusion injury. The effect of Tanshinone on spinal cord ischemia reperfusion injury in rabbit model were achieved by declinedTNF-α, IL- 1β,and increased IL-1Ra. Moreover, Time-lapse effect was observed in the test.