乳腺癌及其癌前病变中细胞凋亡与p53、bcl-2蛋白的表达

Expression of Apoptosis and p53, bcl-2 Protein in Breast Cancer and Its Precancerous Lesions

目的 :通过观察乳腺癌及其癌前病变中细胞凋亡和凋亡调控基因 p5 3、bcl- 2的表达 ,探讨细胞凋亡与凋亡调控基因在乳腺组织恶性转化进程中的作用。方法 :利用DNA缺口末端标记技术和免疫组织化学染色 ,原位观察 31例乳腺癌 ,2 0例乳腺不典型增生和 2 0例乳腺单纯性增生中细胞凋亡和p5 3、bcl- 2蛋白的表达 ,以 8例正常乳腺组织作为对照。结果 :乳腺不典型增生和单纯性增生中细胞凋亡指数显著高于乳腺癌及正常乳腺组织 (P <0 0 1) ,乳腺癌中细胞凋亡指数高于正常乳腺组织 (P <0 0 5 )。乳腺癌和乳腺不典型增生中 p5 3蛋白阳性率分别高于正常乳腺组织 (P <0 0 5 )。凋亡细胞多分布在 p5 3、bcl- 2蛋白阴性区 ,阳性区仅有少量分布 ,且 p5 3、bcl- 2蛋白阴性组细胞凋亡指数高于阳性组 (P <0 0 5 )。结论 :细胞凋亡调控失调在乳腺组织恶性转化进程中起重要的作用。突变型p5 3、bcl- 2蛋白可抑制细胞凋亡。

Purpose: To investigate the effects of apoptosis and its regulating genes in malignant transformation of breast cancer and its precancerous lesions Methods: The expression of apoptosis and the positive expression of p53, bcl-2 proteins were observed in 31 cases of breast cancer in situs, 20 breast dysplasia and 20 breast hyperplasia by using DNA nick end labeling (TUNEL) technique and immunohistochemical staining Eight normal breast tissues were used as control Results: The apoptotic indexes in breast dysplasia and breast hyperplasia were significantly higher than those in breast cancer and normal ( P< 0 01) The apoptotic index in breast cancer was higher than those in normals ( P< 0 05) The positive expression rate of p53 protein in breast cancer and dysplasia were higher than that in normal control ( P< 0 05) The majority of apoptotic cells were located in the negative expression area of p53, bcl-2 proteins, a few of apoptotic cells were located in the positive area The apoptotic index in negative group of p53, bcl-2 proteins was higher than that in positive group ( P< 0 05) Conclusion: The abnormal regulation of apoptosis may play an important role in malignant transformation of breast cancer,and p53, bcl-2 proteins may inhibit apoptosis