斜纹夜蛾核多角体病毒郴州株的生物学与应用研究

Biology and Application of Spodoptera litura nucleopolyhedrovirus Chenzhou Strain

本研究首次在湖南郴州分离出了斜纹夜蛾核多角体病毒(Spodoptera litura nucleopolyhedrovirus,SpliNPV)郴州株,并对其室内生物活性分析和田间应用效果进行了系统研究。结果表明,该病毒的人工繁殖方法以饲喂法较好,病死率为65.0%;害虫染毒后4～8 d达死亡高峰,高温、高湿、寡日照天气有利于病毒的发生流行;此病毒的室内校正防效为86.6%,略优于已投入市场的病毒产品;田间校正防效为88.4%,稍高于阿维菌素农药产品的防效;病毒原液中的病毒多角体的浓度为1.03×1011 OBs/mL,为国内的强毒株。该病毒作为防治害虫的生物因子,具有很好的应用开发前景。

The laboratory bioassay and field control efficacy of Spodoptera litura nucleopolyhedrovirus（SpliNPV） Chenzhou strain were preliminarily examined.The efficient artificial propagation method was used to feed the host larvae with virus suspension,the average mortality of the insects was 65.0%.The death peak of the pests was in 4-8 days after infecting the virus.The high temperature and humidity,and poor light could help this virus.Filed control efficacy of Chenzhou strain was 86.6% in laboratory,which was better than another commercial strain.The corrected control efficacy of this strain was 88.4% in the fields,which was higher than that of avermectin pesticide significantly.It was detected that the occlusion body（OB） concentration of the initial virus＇s stock solution was 1.03 ×1011 OBs/ ml,and it was a strong SpliNPV stain,as it showed an excellent efficacy to control the pest Spodoptera litura,there will be a good prospect of application and development of this SpliNPV strain.