麦纤散对大鼠溃疡性结直肠癌胰岛素样生长因子结合蛋白7及细胞凋亡影响

Influences of Maixiansan on insulin-like growth factor binding protein7 and apoptosis in rats with ulcerative colitis-related colorectai cancer

目的探讨麦纤散对溃疡性结肠炎相关性结直肠癌实验大鼠结肠组织胰岛素样生长因子结合蛋白7（insulin-like growth factor binding protein 7,IGFBPT）基因表达及结肠肿瘤细胞凋亡的影响。方法将40只SD大鼠随机分为4组：空白组、模型组、麦纤散组与美莎拉嗪组；除空白组外，其余3组使用葡聚糖硫酸钠联合氧化偶氮甲烷的化学方法建立溃疡性结肠炎相关性结直肠癌大鼠模型，并分别应用药物干预，于16周后处死取结直肠，采用原位末端标记法（TUNEL）和实时荧光定量PCR技术分别检测大鼠结直肠肿瘤细胞凋亡率及结肠组织IGFBP7基因表达。结果结肠肿瘤细胞凋亡指数麦纤散组（8．70±3．47）和美莎拉嗪组（1．20士0．26）与模型组（O．38土0．11）比较均提高，麦纤散组与模型组比较，差异有统计学意义（P〈0．05）；麦纤散组结肠组织IGFBP7基因表达为50．5±14．0，与模型组18．0±3．9比较明显增加，差异有统计学意义（P〈0．05）。结论麦纤散能对抗溃疡性结肠炎相关性结肠癌的发生与发展，其机制与促进肿瘤细胞凋亡、增加结肠组织IGFBP7表扶有关。

Objective To investigate the effects of Maixiansan on insulin like growth factor binding protein 7（IGFBP7） and apoptosis in rats with ulcerative colitis-related colorectal cancer. Methods The rat model of ulcerative colitis-related colorectal cancer was induced by dextran sulfate sodium （DSS） and azoxymethane（AOM）. 40 male SD rats 〈weight （160 ± 10）g] were randomly divided into 4 groups： model, Maixiansan and Meisalazine treatment as well as normal group peritoneally injected with saline. The expression of IGFBP7 and apoptosis in colorectal tissue were detected by real time PCR and TUNEL after 16 weeks. Results The numbers of colorectal cancer in model group（1.2±0.4）, in Maixiansan group（0.70±0.15）, in Meisalazine group（0.60± 0.16） were higher than in normal control （P 〈 0.05）, but no differences were found among model, Maixiansan and Meisalazine groups（P〉0.05）. The apoptosis in colonic mucosa for Meixiansan（8.70±3.47） group and Mesalazine group were enhanced as compared with that in model group（1.20! 0.26 vs. 0. 38±0.11, P〈0.05）. The mRNA expression of IGFBP7 in colon for Meixiansan group were higher than those in model group, Meisalazine group, and normal control（50.5 ± 14.0 vs. 18.0 ± 3.9 and 39.3± 11.4, 46.4±6.0, P〈0.05）. Conclusions Maixiansan may resist the occurrence and development of ulcerative colitisrelated colorectal cancer through upregulating IGFBP7 expression of colorectal tissue and promoting apoptosis of tumor cell.