超微粉碎对银杏叶中总黄酮溶出量的影响

Effect of Ultrafine Grinding on the Dissolution of Total Flavonoids in Ginkgo biloba Leaves

[目的]研究超微粉碎对银杏叶中总黄酮溶出的影响。[方法]利用扫描电镜对银杏叶细粉和超微粉进行观察;采用分光光度法测定银杏叶细粉和超微粉中总黄酮溶出量。[结果]银杏叶超微粉在电镜下观察难以看到完整的细胞结构,绝大多数细胞破壁;银杏叶超微粉中总黄酮溶出量比细粉高(P<0.05);在超声波提取或简化提取(开水冲搅)下,银杏叶超微粉中总黄酮溶出量分别比细粉提高了10.0%和11.8%(P<0.05)。[结论]超微粉碎可提高银杏叶中总黄酮溶出量。

[Objective] The aim was to study the effect of ultrafine grinding on the dissolution of total flavonoids in G.biloba leaves.[Methods] Scanning electron microscope was used to observe the fine powder and superfine powder of G.biloba leaves,and the content of flavonoids in the fine powder and superfine powder of G.biloba leaves was determined by spectrophotometry.[Result] It was difficult to observe an integrated cell structure of G.biloba leaves superfine powder in scanning electron microscope,and the great majority of the cells were broken.The superfine powder of G.biloba leaves showed a higher dissolution rate of flavonoids than the fine powder（P0.05）.Compared with the fine powder,the content of flavonoids in the superfine powder of G.biloba leaves was increased by 10.0% under ultrasonic extraction and 11.8% under simple extraction（stirred by spoiling water）（P0.05）,respectively.[Conclusion] Ultrafine grinding could improve the dissolution of flavonoids in G.biloba leaves.