肾上腺醛固酮腺瘤大鼠模型的建立

Establishment of a Rat Model of Aldosterone-Producing Adenoma

目的构建肾上腺醛固酮腺瘤大鼠模型.方法 16只SD大鼠随机分对照组和醛固酮腺瘤组,在ALZET 2004微量渗透泵储药仓内分别注入醛固酮溶液或空白溶剂,然后将其埋于大鼠背部皮下.ALZET 2004微量渗透泵可以持续灌注4周,为使醛固酮作用时间达到8周,4周后更换渗透泵1次,同时尾套法测量大鼠尾动脉收缩压;8周后,放免法检测血浆醛固酮浓度和肾素活性.结果 3周后,与对照组大鼠相比,腺瘤组大鼠收缩压开始升高,第7周达到顶峰,之后维持在高水平;腺瘤组大鼠血浆醛固酮的浓度显著升高(P<0.01),肾素活性被抑制(P<0.01).结论大鼠皮下埋植微量渗透泵灌注醛固酮,可以成功构建大鼠醛固酮腺瘤模型.

Objective To establish a rat model of aldosterone-producing adenoma（APA）.Methods 16 Sprague-Dawley rats subcutaneously implanted with an osmotic mini-pump,were randomly divided into 2 groups： control group（vehicle）and APA（1 μg/h）group.Because the mini-pump（ALZET 2004）could continuously infuse test substance for 4 weeks,another minipump was replaced after the fourth week to make the duration of aldosterone infusion for 8 weeks.Systolic blood pressure（SBP）was measured weekly by the tail-cuff method.At the termination of the study,blood was collected for measurements of plasma renin activity（PRA）and aldosterone concontration（PAC）with radioimmunoassay kits.Results After 3 weeks,APA rats showed significantly and progressively increased SBP compared with controls（P0.05）,and the SBP level reached peak on 7th week and remained high levels.PRA was substantially depressed and PAC was significantly raised in APA rats（P0.01）.Conclusion By implanting osmotic minipumps subcutaneously,the rat models of APA can be successfully established,which provide a good platform for investigating the pathogenesis of APA.