近红外量子点单克隆抗体荧光探针对U14头颈部移植鳞癌的原位可视化成像研究

In situ visual imaging of U14 head and neck squamous cell carcinoma xenograft tumors by near-infrared quantum dots-monoclonal antibody fluorescent probes

目的:观察近红外荧光量子点(Near-infrared fluorescent quantum dots,NIRF-QDs)表皮生长因子受体单克隆抗体(Epi-dermal growth factor receptor monoclonal antibody,EGFR mAb)荧光探针对U14头颈部移植鳞癌的原位可视化成像情况。方法:将EGFR mAb偶联发射波长为800 nm的近红外荧光量子点(Quantum dot,QD800)制备近红外量子点表皮生长因子受体单克隆抗体荧光靶向探针(QD800-EGFR mAb),将高表达表皮生长因子受体的U14鳞癌细胞和QD800-EGFR mAb共培养,观察QD800-EGFR mAb与U14细胞表达的EGFR结合情况;建立U14头颈部移植鳞癌裸鼠模型,通过尾静脉注射QD800-EGFRmAb,观察对U14头颈鳞癌活体原位可视化动态成像情况。结果:体外实验证明:QD800-EGFR mAb能与U14细胞表达的EGFR特异性结合;体内活体成像显示:QD800-EGFR mAb静脉注射后能对U14头颈部移植鳞癌进行清楚的原位可视化荧光成像,荧光成像一直持续到24 h,在1～6 h时间段内肿瘤成像最完整和信噪比最高。结论:基于以EGFR为靶点的NIRF-QDs技术对头颈部鳞癌的发生发展、原位可视化成像和个体化治疗具有独特的优势和发展前景。

Objective：To study the in situ visual imaging of near-infrared quantum dots（NIRF-QDs） epidermal growth factor receptor monoclonal antibody（EGFR mAb） fluorescent probes on the U14 head and neck squamous cell carcinoma xenograft tumors.Methods：Epidermal growth factor receptor monoclonal antibodies were coupled to near-infrared fluorescent quantum dots（QD800） to prepare near-infrared fluorescent quantum dots-EGFR mAb probes（QD800-EGFR mAb）.The squamous cell carcinoma U14 with high expression of EGFR and QD800-EGFR mAb were cocultured to observe the combination in vitro;serial visual in vivo imaging of U14 head and neck squamous cell carcinoma xenograft tumors was done after intravenous injection of QD800-EGFR mAb.Results：Experiments in vitro showed that QD800-EGFR mAb could bind specifically to U14 cells.In vivo imaging showed that clear visual in situ fluorescence imaging of head and neck squamous cell carcinoma could be achieved after intravenous injection of QD800-EGFR mAb,and the fluorescence imaging lasted up to 24 h during which the most complete and highest signal to noise ratio of tumor in vivo imaging was in 1～6 h.Conclusion：EGFR target based near near-infrared quantum dots technique has unique advantages and prospects for the occurrence and development study,visual in situ imaging and individual treatment of head and neck squamous cell carcinoma.