HDV/HBV感染树鼩肝组织Fas/FasL表达与肝细胞凋亡

Fas/ FasL expression and hepatocyte apoptosis on liver tissues in tupaia with HDV/HBV infection

目的 探讨ＨＤＶ感染树Ｑｕ肝组织中Ｆａｓ／ＦａｓＬ表达与ＨＤＶ感染之间的关系，以及Ｆａｓ／ＦａｓＬ在丁型肝炎细胞凋亡中的作用。方法 采用免疫组化和原位杂交对４５份ＨＤＶ感染树Ｑｕ肝中ＨＤＡｇ，Ｆａｓ／ＦｑｓＬ和Ｆａｓ／ＦａｓＬｍＲＮＡ的表达进行了检测；应用原位末端标记技术对肝细胞凋亡进行了检测，并应用免疫组化双重染色对ＨＤＡｇ，Ｆａｓ／ＦａｓＬ的表达以及肝细胞凋亡进行了检测。

AIM To investigate the relationship between expression of Fas /Fas ligand (FasL) and HDV infection, and its role in hepatocyte apoptosis in tupaia. METHODS Fourty-five cases of tupaia liver tissues with HDV infection were studied by immunohistochemistry and in situ hybridization to determine HDAg, Fas/ FasL and Fas/ FasL mRNA, by in situ end-labeling to determine hepatocyte apoptosis, and by immunohistochemical double labeling techniques to determine HDAg, Fas, FasL and apoptosis.RESULTS Fas and FasL were detected in 39 (87%) of 45 liver uissue specimens and hepatocyte apoptosis in 41 (91%). There was a significant correlation between expression of HDAg and Fas/ FasL ( x12= 29.2, x22 = 27.9, P<0.05). The more the expression of HDAg was, the more the expression of Fas/ FasL was. Apoptosis could occur in both HDAg positive and negative cells, but mainly occur in HDAg positive cells. Fas/ FasL expression was closely correlated to the hepatocyte apoptosis (x12 = 35.1, x22= 40.2, P<0.05). The more expression of Fas/FasL was, the more hepatocyte apoptosis was. CONCLUSION Apoptosis can occur in both the viral infected and uninfectedd hepatocytes, but only in a minority of hepatocytes. Fas expression may be induced by HDAg in hepatocyte. Fas/ FasL may play an important role in hepatocyte apoptosis in HDV infection.