Molecular Cloning of IGFBP-1 Gene and Developmental Expression of Its mRNA in Different Tissues of Nanjiang Mongolian Gazelles

The objective of the present study was to investigate the developmental expression patterns of Insulin-like growth factor-binding protein 1 (IGFBP-1) gene in different tissues of postnatal Nanjiang Mongolian Gazelles. Samples of heart, liver, spleen, lung, longissimus dorsi, semimembranosus, m. triceps brachii and biceps muscle of thigh were collected from a total of 36 Nanjiang Mongolian Gazelles at the age of 0, 15, 30, 60, 90 and 120 days after birth (3 males and 3 females at each age). The CDS was sequenced and ontogeny of mRNA levels of IGFBP-1 were measured by real-time fluorescence quantitative RT-PCR. The size of IGFBP-1 ORF was 792 bp encoding 263 amino acid residues, and displayed higher nucleotide/amino acid sequence identities with other ruminants compared to non-ruminants. The levels of IGFBP-1 mRNA in liver were highest (P<0.01), levels were medium in lung, spleen and heart, and the lowest in the muscles; there were no significant differences among the muscles (P>0.05). Three expression patterns of IGFBP-1 mRNA during postnatal growth from birth to day 60 were found: consistently decreasing (liver), fluctuating as increasing then decreasing (heart) or as decreasing then increasing then decreasing (spleen, lung and muscles). The results indicate that the IGFBP-1 gene is highly conserved among species, and liver has the highest expression. It was concluded that IGFBP-1 plays important roles in early postnatal growth and is expressed in a developmental-tissue-dependent manner.

The objective of the present study was to investigate the developmental expression patterns of Insulin-like growth factor-binding protein 1 （IGFBP-1） gene in different tissues of postnatal Nanjiang Mongolian Gazelles. Samples of heart, liver, spleen, lung, longissimus dorsi, semimembranosus, m. triceps brachii and biceps muscle of thigh were collected from a total of 36 Nanjiang Mongolian Gazelles at the age of 0, 15, 30, 60, 90 and 120 days after birth （3 males and 3 females at each age）. The CDS was sequenced and ontogeny ofmRNA levels of IGFBP-1 were measured by real-time fluorescence quantitative RT-PCR. The size ofIGFBP-10RF was 792 bp encoding 263 amino acid residues, and displayed higher nucleotide/amino acid sequence identities with other ruminants compared to non-ruminants. The levels oflGFBP-1 mRNA in liver were highest （P〈0.01）, levels were medium in lung, spleen and heart, and the lowest in the muscles; there were no significant differences among the muscles （P〉0.05）. Three expression patterns of 1GFBP-1 mRNA during postnatal growth from birth to day 60 were found： consistently decreasing （liver）, fluctuating as increasing then decreasing （heart） or as decreasing then increasing then decreasing （spleen, lung and muscles）. The results indicate that the IGFBP-1 gene is highly conserved among species, and liver has the highest expression. It was concluded that IGFBP-1 plays important roles in early postnatal growth and is expressed in a developmental-tissue-dependent manner.