不同产地菟丝子总黄酮及金丝桃苷的含量测定

Quantitative Determination of Total Flavones and Hyperoside in Cuscuta Chinensis Lam Collected from Different Places

[目的]用紫外可见分光光度法测定不同产地(宁夏、内蒙古、辽宁、江苏、河北、陕西、四川、山东)菟丝子中总黄酮的含量;用高效液相色谱(HPLC)法测定金丝桃苷的含量。[方法]紫外可见分光光度法采用NaNO2-Al(NO3)3-NaOH体系显色,以芦丁为对照,在504nm波长处测定吸光度;HPLC法采用Ultimate XB-C18色谱柱(250mm×4.6mm,5μm),流动相:乙腈-0.1%磷酸溶液(17:83),流速:1.0 mL.min-1,柱温:30℃,检测波长:360nm。[结果]不同产地菟丝子药材中的总黄酮含量最高为2.55%,最低为0.38%;金丝桃苷含量最高为0.276%,最低为0.012%。[结论]不同产地菟丝子药材中总黄酮及金丝桃苷含量均存在较大差异,且总黄酮含量越高,金丝桃苷含量相应也高,二者呈一定相关性。

[Objective] Ultraviolet-Visible spectrophotometer（UV） and HPLC methods were developed to determine the content of total flavones and hyperoside in Cuscuta chinensis Lam collected from different places（Ningxia,Neimenggu,Liaoning,Jiangsu,Hebei,Shanxi,Sichuan and Shandong）. [Methods] Rutin was used as the standard to measure the total flavones,the colour reactoin was carried out with NaNO2-Al（NO3）3-NaOH and measu-red in the 504 nm wavelength by UV spectrophotometry.The hyperoside was separated with acetonitrile-0.1%H3PO4（17：83）（flow rate set at 1.0 mL·min-1,wavelength was 360 nm） by Ultimate XB-C18（250 mm×4.6 mm,5 μm） column.[Results] The total flavones contained in Cuscuta chinensis was from 0.38% to 2.55%;the amounts of hyperoside was from 0.012% to 0.276%.[Conclusion] The amounts of both total flavones and hyperoside were of significantly different Cuscuta chinensis Lam from different places,and there was a significant correlation between the contents of total flavones and the content of the hyperoside in the same sample： namely the higher the level of the total flavones,the higher the content of hyperoside.