^(99)Tc^m-抗血栓成分单克隆抗体对动物血栓的放免显像研究

Radioimmunoscintigraphy of Experimental Arterial and Venous Thrombi in Animals with 99 Tc m Labeled Monoclonal Antibody Against Thrombus Elements

目的 评价９９Ｔｃｍ 标记抗血栓成分（ 血小板和纤维蛋白）的单克隆抗体（ＭｃＡｂ） ＳＺ－ ５１ 和ＳＺ－ ６３，对实验性动物（ 兔、狗）股动脉和股静脉血栓模型显像的作用。方法 以亚氨基噻吩盐酸盐（２ － ＩＴ） 修饰，９９Ｔｃｍ 标记葡庚糖酸钠（ＧＨ） 配体交换法标记ＳＺ－ ５１ 和ＳＺ－ ６３。狗、兔股动脉和股静脉血栓模型制备完毕后，耳缘静脉注入９９ Ｔｃｍ － ＳＺ－ ５１或９９Ｔｃｍ － ＳＺ－ ６３ 进行ＳＰＥＣＴ 显像。９９Ｔｃｍ 标记非特异性鼠ＩｇＧ 作为阴性对照。结果 ９９Ｔｃｍ － ＳＺ－５１ 和９９Ｔｃｍ － ＳＺ－５１／６３ 对狗新鲜血栓均显示清晰，其最佳显像时间分别为注射后２ ～４ｈ 和０．５ ～２ｈ，而陈旧血栓未见显像。９９Ｔｃｍ － ＳＺ－ ６３ 兔陈旧血栓显示清晰，其最佳显像时间为注射后２ ～４ｈ。结论 ９９Ｔｃｍ － ＳＺ－ ５１ 和９９Ｔｃｍ － ＳＺ－ ６３ 具有活体内血栓定位导向能力，显像时间短，用于血栓性疾病的放免显像有一定的可行性。

Object To evaluate the use of 99 Tc m labeled anti P-Selection monoclonal antibody (McAb)SZ-51 and anti-fibrin McAb SZ-63 in detection of experimental thrombi in rabbits and dogs.Method The McAb SZ-51 and SZ-63 were labeled by using the method of 2-iminothiolane modification and 99 Tc m-glucoheptonate(GH) transchelation.The experimental femoral arterial and venous thrombosis were prepared,then 99 Tc m-McAb was injected into ear-edge vein,finally imaged by SPECT. 99 Tc m-labelled murine IgG was used as a negative control.Results The fresh arterial and venous thrombi in dogs were clearly imagded 0.5 to 2h and 2 to 4h after injection of 99 Tc m-SZ-51/63 and 99 Tc m-SZ-51,respectively.The old arterial and venous thrombi in rabbits were clearly imaged 2 to 4h after injection of 99 Tc m-SZ-63.Conclusion The monoclonal antibody SZ-51 and SZ-63 would be a potential agent for imaging diagnosis of thrombotic disease.