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中草药提取液对流感病毒诱导细胞凋亡的影响 被引量:9

Effects of Chinese Herbal Extracts on Cell Apoptosis Induced by Influenza Viruses
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摘要 目的探讨金银花、黄芩和连翘的提取液对甲3型流感病毒诱导狗肾传代细胞(MDCK细胞)凋亡的影响。方法采用金银花、黄芩和连翘3种中草药的提取液进行抗病毒试验,以细胞病变、姬姆萨染色在光镜下观察细胞形态学变化,Annexin V和PI双染用流式细胞仪测定细胞凋亡率,评价药物对甲3型流感病毒诱导MDCK细胞凋亡的影响。结果在MDCK细胞上甲3型流感病毒(血凝滴度为1∶64)的TCID50为0.65.100μL-1;实验浓度:金银花为1∶256(1.95g.L-1),黄芩为1∶64(7.81g.L-1),连翘为1∶64(7.81g.L-1)。在光镜下观察甲3型流感病毒对照组的MDCK细胞,可见被感染的MDCK细胞出现了细胞凋亡特有的形态学变化。Annexin V和PI双染经流式细胞仪测定显示3种药物实验组的细胞凋亡率[金银花(28.13±3.30)%、黄芩(13.47±3.04)%、连翘(19.17±6.55)%]均显著低于病毒对照组[(54.10±4.58)%](P<0.001)。结论 7.81g.L-1的黄芩及7.81g.L-1的连翘均能抑制100TCID50的甲3型流感病毒诱导MDCK细胞凋亡。1.95g.L-1的金银花不能够有效抑制100TCID50的甲3型流感病毒诱导MDCK细胞凋亡。 Objective To investigate the effects of lonicerae,scutellaria and forsythiae extracts on influenza virus A3-induced apoptosis in Madin-Darby canine kidney(MDCK) cells.Methods The effects of lonicerae,scutellaria and forsythiae on influenza virus A3-induced MDCK cell apoptosis were investigated to assess the anti-virus activities of Chinese herbal extracts.Cytopathy was determined by Giemsa staining under the light microscope and cell apoptosis was detected using flow cytometry by double-labeling with Annexin V and PI.Results The TCID50 of H3N2 influenza virus(hemagglutinin titer of 1∶64) was 0.65·100 μL-1 in MDCK cells.Experimental concentrations of lonicerae,scutellaria and forsythiae were 1∶256(1.95 g·L-1),1∶64(7.81 g·L-1) and 1∶64(7.81 g·L-1),respectivelty.The infected MDCK cells showed apoptosis-specific morphological changes under the light microscope.Flow cytometry showed that lonicerae,scutellaria and forsythiae significantly decreased the apoptotic rates compared with control virus-infected cells [(28.13±3.30)%,(13.47±3.04)% and(19.17±6.55)% vs(54.10±4.58)%,respectively;P0.001].Conclusion Scutellaria extract(7.81 g·L-1) and forsythiae extract(7.81 g·L-1) can inhibit influenza virus A3-induced MDCK cell apoptosis,but lonicerae extract(1.95 g·L-1) has no significant inhibitory effect on MDCK cell apoptosis.
作者 刘晓静 孙坚
出处 《南昌大学学报(医学版)》 CAS 2012年第1期10-13,共4页 Journal of Nanchang University:Medical Sciences
基金 江西省卫生厅中医药科研基金(2009A024)
关键词 中草药 金银花 黄芩 连翘 流感病毒 细胞凋亡 流式细胞术 Chinese herbals lonicerae scutellaria forsythiae influenza virus apoptosis flow cytometry
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  • 1Oca(n)a Macchi M,Bel M,Guzylack Piriou L. Hemagglutinin-dependent tropism of H5N1 avian influenza virus for human endothelial cells[J].Journal of Virology,2009,(24):12947-12955.
  • 2Nelson M I,Edelman L,Spiro D J. Molecular epidemiology of A/H3N2and A/H1N1 influenza virus during a single epidemic season in the United states[J].PLoS Pathogens,2008,(08):e1000133.doi:10.1371/journal.ppat.1000133.
  • 3Tran T H,Nguyen T L,Nguyen T D. Avian influenza A (H5N1) in 10 patients in Vietnam[J].New England Journal of Medicine,2004,(12):1179-1188.
  • 4蔡柏蕾;李龙芸.协和呼吸病学[M]北京:中国协和医科大学出版社,2005586-596.
  • 5胡仁火.五种中草药提取液对细菌作用效果初探[J].咸宁学院学报,2006,26(6):133-135. 被引量:11
  • 6孙坚,王农荣,杨斌,何士勤,刘晓静.双黄连抑制甲1型流感病毒诱导细胞凋亡的机制研究[J].中国药房,2007,18(27):2086-2088. 被引量:14

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