摘要
目的 :研究炎症递质在兔心肌缺血再灌注损伤中的作用。方法 :采用兔心肌缺血再灌注模型观察血浆白细胞介素 - 6 (IL- 6 )、白细胞介素 - 8(IL- 8)的变化 ,外周血中性粒细胞表面 CD1 1 b表达、电镜下的形态以及心肌组织中髓过氧化物酶 (MPO)活性和丙二醛 (MDA)含量。结果 :单纯缺血和缺血再灌注血浆 IL- 6、IL- 8,中性粒细胞表面 CD1 1 b表达和心肌缺血区 MPO活性、MDA含量均显著高于正常对照 (P <0 .0 1或 <0 .0 5 )。 1h再灌注组的上述指标比 1.5 h缺血组显著性增高 ,4h再灌注组较 1h再灌注组进一步升高 (P <0 .0 1)。 4h再灌注组于再灌注前静脉注射 IL - 6单克隆抗体 (IL - 6 Mc Ab)后 ,上述指标显著性降低 (P <0 .0 1或 <0 .0 5 ) ,梗死范围缩小 37.2 %(P <0 .0 1)。 IL - 6、IL - 8、CD1 1 b与 MPO活性呈正相关 ,IL - 6、IL - 8与 CD1 1 b也呈正相关。电镜下缺血再灌注时周围血中性粒细胞呈活化表现。结论 :炎症递质 IL - 6、IL - 8参与了心肌缺血再灌注损伤的发生。抑制炎症递质的作用 。
Objective:[WT9.,8.5BZ]To investigate the effects of inflammatory mediators in myocardial ischemia reperfusion injury.[WT9.,8.5HZ]Methods:[WT9.,8.5BZ]In the rabbit model with myocardial ischemia reperfusion (MI/R),the changes of plasma levels of interleukin 6 (IL 6) and interleukin 8 (IL 8) by ELISA,myeloperoxidase (MPO) activity and MDA content by fluorometry in the myocardium,and the CD 11b expression of circulating neutrophils by flow cytometry were determined.Meantime,the state of circulating neutrophils were observed under electromicroscrope.[WT9.,8.5HZ]Result:[WT9.,8.5BZ]Plasma levels of IL 6 and IL 8,the CD 11b expression of circulating neutrophils,MPO activity and MDA content of myocardial ischemic area were higher in myocardial ischemia and ischemia reperfusion groups than those in the control group (P< 0.05 or P< 0.01 ).These indexes were significantly increased in R1h group than those in I 1.5 h group,and further elevated in R4 h group than those in R1 h group.With the intervention of IL 6 monoclonal antibody before reperfusion of ischemic myocardium in R4 h group (n=8),necrotizing area was significantly decreased (P< 0.001 ),and MPO activity,the CD 11b expression of circulating neutrophils and MAD content of myocardial ischemic area (P< 0.05 or P< 0.01 ) decreased too.Plasma levels of IL 6 and IL 8,the CD 11b expression of circulating neutrophils.[WT9.,8.5HZ]Conclusion:The activating state of circulating neutrophils with the pseudopodia,degranulation and vesicle formation were observed.Inflammatory mediators such as IL 6 and IL 8 might participate the pathogenesis of myocardial ischemia reperfusion injury.The blockade of inflammatory mediators might attenuate myocardial ischemia reperfusion injury.correlated positively with MPO activity of myocarddial ischemic area.Plasma leveals of IL 6 and IL 8 correlated positively with the CD 11b expression of circulating neutrophils. [WT9.,8.5HZ]
出处
《临床心血管病杂志》
CSCD
北大核心
2000年第2期85-87,共3页
Journal of Clinical Cardiology
基金
国家教委博士点基金资助 !(No.95 0 10 30 )
关键词
再灌注损伤
心肌缺血
IL-6
IL-8
Myocardial ischemia reperfusion injury Interleukin 6 Interleukin 8