RT-PCR技术在南方虫媒病毒流行病学调查中的应用

Investigation on the Distribution of Arbovirus in the South of China by Reverse Transcription Polymerase Chain Reactions

目的 :调查南方地区虫媒病毒感染情况。方法 :应用虫媒病毒的通用引物逆转录 -聚合酶链反应技术 (RT -PCR)。结果 :检测 110组骚扰阿蚊、90组白纹伊蚊和 18组致倦库蚊标本 ,分别检出 15组、9组和 2组阳性标本 ,阳性率分别为 13.6 3%、10 %、11.11% ;16 8份发烧病人待查标本中 ,2 8份疑似登革热血标本、11份疑似乙型脑炎血标本和 3份“不明热”血标本 ,均扩增出4 13bp的特异带 ,证实为虫媒病毒感染。结论 :①我国南方地区存在除登革病毒和乙型脑炎病毒外的其它黄病毒感染 ;② 3种蚊虫标本检测出的阳性率表明 ,还可能存在新虫媒病毒疫源地。

Aim:To investigate the distribution of arbovirus in the south of China. Methods: Serum samples from 168 patients with unkown fever and the samples mosquitoes were assayed by reverse transcription polymerase chain reaction (RT-PCR). Results: The 413bp specific fragment was amplified in 15 of 110 group samples mosquitoes Aedes subalbatus , 9 of 90 group Aedes albopictus and 2 of 18 group Culex quinquefasciatus . The positive rates were 13.63%,10% and 11.11% repectively. Serum sample from 28 patients with suspected dengue fever, from 11 patients with JEV and from 3 patients with unkown fever was also amplified fragments about 413bp in length. Conclusion: It suggested that there exist more flavivirus infection than DEN and JEV in south China. And from the positive rates of three kinds of mosquitoes samples, we concluded that there perhaps are new arbovirus foci.