拉曼光谱研究丹参注射液对人红细胞膜作用及机制讨论

Raman spectroscopic study on effect of Danshen injection on human erythrocyte membranes and its mechanism

作为活血化瘀传统中药,丹参具有改善红细胞的变形性的显著临床作用,其对红细胞膜直接作用目前尚不清楚。本研究采用显微共聚焦拉曼技术,测量丹参注射液作用前后人红细胞的拉曼光谱变化。结果显示丹参注射液作用后红细胞膜部分谱线对应的基团构象发生微小变化,归属于磷脂分子脂酰基C-C骨架反式构象的1 064,1 126cm-1谱线强度明显减弱,归属于磷脂分子脂酰基C-C骨架扭曲构象的1 091 cm-1谱线强度明显增强,磷脂链内纵向有序性参数(Strans)明显变小,提示红细胞胞膜在丹参注射液作用下胞膜磷脂分子脂酰基C-C骨架的反式构象转变为扭曲构象,增强红细胞膜磷脂流动性,增强细胞膜的流动性,丹参注射液对红细胞膜结构和功能的改善可为其发挥活血化瘀的中药药理作用的可能内在机制。

Objective： Danshen, as a traditional Chinese medicine for promoting blood circulation and removing blood stasis, is widely applied in improving human erythrocyte deformability in clinics. But its direct effect on erythrocyte membranes is still unclear. Method ： In this essay, the confoeal Raman technique was adopted to measure the changes in Raman spectra of human erythrocytes before and after the administration of Danshen injection. Result： The results showed slight changes in group conformations corresponding to erythroeyte membranes after the administration of Danshen injection. Specifically, 1 064, 1 126 cm^- 1 spectral lines attributed to phospholipid molecule acyl C-C skeleton anti-conformation were obviously weakened, whereas 1 091 cm ^-1 spectral line attributed to phospholipid molecule acyl C-C skeleton guache conformation notably intensified. Besides, the longitudinal order-parameter in chains （ Strans ） of phospholipids was reduced significantly. Conclusion： Danshen injection can transfer erythrocyte membrane phospholipid molecule acyl C-C skeleton anti-conformation to guaehe conformation, indicating the increase in liquidity of erythrocyte membrane phospholipids and erythrocytes. Danshen injection＇s effect in improving erythrocyte membrane structure and function may be the intrinsic mechanism for its pharmacological effect as a traditional Chinese medicine.