ICAM-1 K469E基因多态性与经皮腔内冠状动脉成形术后再狭窄发生的相关性研究

Correlation between polymorphism of ICAM-1 K469E gene and restenosis after percutaneous transluminal coronary angioplasty

目的:研究细胞间黏附分子-1(ICAM-1)K469E基因多态性与经皮腔内冠状动脉成形术(percu-taneous transluminal coronary angioplasty,PTCA)术后再狭窄发生的相关性。方法:收集冠状动脉支架术后行冠状动脉造影随访的患者198例,分为再狭窄组(n=78)和未狭窄组(n=120),采用聚合酶链式反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)方法确定ICAM-1K469E基因位点的基因型,并且比较两组基因型频率和等位基因频率。结果:再狭窄组中KK、EK、EE基因型频率分别为56.41%、28.20%、15.38%,未狭窄组KK、EK、EE基因型频率分别为40.00%、31.67%、28.33%,两组3种基因型的分布差异有统计学意义(χ2=6.34,P<0.05)。再狭窄组中K、E等位基因频率分别为70.51%、29.49%,未狭窄组中K、E等位基因频率分别为55.83%、35.83%,再狭窄组等位基因K和E频率明显高于未狭窄组,差异有统计学意义(χ2=8.61,P<0.01)。结论:ICAM-1K469E基因位点多态性与PTCA术后发生再狭窄存在相关性。

Objective： To study the relationship between the polymorphism of intercellular adhesion molecule-1（ICAM-1） K469E gene and restenosis after percutaneous transluminal coronary angioplasty （PTCA）. Methods： One hundred and ninety-eight patients who underwent PTCA were enrolled and divided into in-stent restenosis group （n=78） and free from restenosis group （n=120）. Polymerase chain reac tion-restriction fragment length polymorphism （PCR-RFLP） was used to detect the genotype of ICAM-1 and genotype and allele frequencies were compared between the two groups. Results：The distribution frequency of genotypes of KK, EK, EE in the ICAM-1 gene were 56.41%, 28.20% and 15.38% in the developed in-stent restenosis group, and 40.00%, 31. 67% and 28. 33% in the free for restenosis group, which were significantly different （x^2 =6.34, P〈0.05）. The frequency of allele K and E was 70.51% and 29.49% in the developed in-stent restenosis group, and 55.83% and 35. 83% in the free for restenosis group showing significant difference （x^2 = 8.61, P〈0.05）. Conclusions. The ICAM-1 K469E gene site polymorphism is related with susceptibility in restenosis after PTCA.