从微藻中提取分离EPA和DHA的方法

Extraction and Purification of EPA and DHA from Microalgae

EPA(eicosapentaenoic acid,EPA)和DHA(docosahexaenoic acid,DHA)由于具有很重要的医疗和保健功效,越来越受到研究者的青睐。与深海鱼相比,微藻材料来源较易,其EPA和DHA的含量更高,是更好的生产EPA和DHA的原料。该研究介绍了几种常用和具有开发潜力的从微藻中提取分离EPA和DHA的方法,包括溶剂提取法、超临界流体萃取法、尿素包合法、高效液相色谱法、超临界流体色谱法和脂肪酶催化反应法,并通过探讨各种方法的优缺点,对其应用前景进行了展望,以期为从微藻中提取分离EPA和DHA的研究提供参考依据。

With important medical and healthcare functions, EPA （eicosapentaenoic acid） and DHA （docosahexaenoicacid） are becoming more and more popular. Compared with the deep-sea fish, the microalgae are easy to get and contain higher content of EPA and DHA. In this paper, several common and potential methods to extract EPA and DHA from the microalgae were introduced, including solvent extraction, supercritical fluid extraction, urea inclusion, high performance liquid chromatography, supercritical fluid chromatography and lipase-catalyzed reactions. Both the advantages and disadvantages of these methods were discussed, and their application prospects were forecasted, aiming to provide basis for the extraction and isolation of EPA and DHA from the microalgae.