黄芪和何首乌抗环磷酰胺诱导胸腺细胞凋亡

Antagonistic Effect of Astragalus mongholicus and Polygonum multiflorum on Thymocytes Apoptosis Induced by Cyclophosphamide

研究了黄芪和何首乌单用和联用对环磷酰胺诱导的胸腺细胞凋亡改变的影响。环磷酰胺对照组 (C组 )的胸腺细胞呈现明显的凋亡改变 ,与AM、PM和AMPM组 (环磷酰胺处理的小鼠加用相应的黄芪、何首乌或黄芪加何首乌 )比较 ,结果表明流式细胞仪检测所出现的反映凋亡细胞数量的亚G1峰 Ap峰 ,各组的高低不一 ,依次为AMPM组 <AM组 <PM组 <C组 ;而正常对照组 (O组 )未出现Ap峰。超微结构显示的凋亡细胞密度和凋亡形态改变的程度 ,各组也依次为AMPM组 <AM组 <PM组 <C组。上述结果表明 ,黄芪和何首乌对环磷酰胺所致的胸腺细胞凋亡改变有拮抗作用 。

The effect of Astragalus mongholicus, Polygonum multiflorum and the combination of Astragalus mongholicus and Polygonum multiflorum on thymocytes apoptosis induced by cyclophosphamide (Cy) was studied. The results were as follows: in the group C (the mice were treated with Cy, Cy mice, the control), their thymocytes revealed obvious apoptosis change, as compared with group C, in group AM, PM and AMPM (the Cy mice were treated for 14 days with Astragalus mongholicus , Polygonum multiflorum and the combination of Astragalus mongholicus and Polygonum multiflorum respectively),using flow cytometry,the sup G1 peaks, labelled ‘Ap’—the hallmark of apoptotic cells appeared, the height of Ap peaks was sequentially in group AMPM < AM < PM < C , and Ap peak did not appear in group O. The ultrastructure showed that apoptotic thymocytes density and degree of morphologic change were sequentially in group AMPM < AM < PM < C. The results suggested that Astragalus mongholicus and Polygonum multiflorum could play an antagonistic role on thymocytes apoptosis induced by cyclophosphamide, and the combination of Astragalus mongholicus and Polygonum multiflorum could give rise to the highest effect.