两产地罗勒挥发油化学成分比较

Studies on Chemical Constituents of Volatile Oil of Ocimum basilicum from Two Different Sources

目的:比较分析广东广州与安徽亳州产罗勒挥发油化学成分差异。方法:用水蒸气蒸馏法提取罗勒挥发油,用气相-质谱联用仪(GC-MS)对挥发油成分进行分析,并通过峰面积归一化法计算各成分的相对百分含量;采用薄层色谱法进行比较分析。结果:广州产罗勒挥发油鉴定出29种化合物,并确定了其相对含量,占总含量的99.90%,主成分为对烯丙基茴香醚(83.082%)、芳樟醇(4.734%)、τ-杜松醇(2.715%)、桉叶油素(2.251%)。亳州产罗勒挥发油鉴定出31种化合物,并确定了其相对含量,占总含量的98.65%,主成分为芳樟醇(26.909%)、肉桂酸甲酯(21.85%)、τ-杜松醇(17.411%)、喇叭茶醇(14.154%);薄层色谱显示广州产罗勒挥发油比亳州产者多了2个特征斑点。结论:产地对罗勒挥发油化学成分具有显著影响,气质联用和薄层色谱法均可用于罗勒挥发油的鉴别和产地分析,提示罗勒开发利用应明确品质产地。

Objective：To analyze the chemical constituents of volatile oil from Ocimum basilicum collected in two different sources.Method： The volatile components were extracted by steam distillation method,and analyzed by GC-MS.Peak area normalization method was used for the relative content of volatile oil.TLC was used for the identification of two samples.Result： Twenty-nine components were identified in O.basilicum from Guangzhou,which accounted for 99.90% of the total relative content.The main components were p-allylanisole（83.082%）,linalool 4.734%）,tau-cadinol（2.715%）,eucalyptol（2.251%）.Thirty-one components were identified in O.basilicum from Bozhou,which accounted for 98.65% of the total relative content.The main components were linalool（26.91%）,methyl cinnamate（21.85%）,tau-cadinol（17.41%）,ledol（14.154%）.Two characteristic spots could not be identified in O.basilicum from Bozhou but from Guangzhou.Conclusion： The volatile oil from different sources had obviously different chemical constituents.GC-MS and TLC can be used for the identification and analysis of sources.It pointed out that O.basilicum should clear about its quality and sources before exploitation and application.