RP-HPLC法筛选千斤拔有效成分的提取工艺

Optimization of the Extraction Technology of the Components in Moghania philippinensis (Merr.et Rolfe) Li by RP-HPLC

研究了千斤拔有效成分提取分离的最佳工艺条件,通过RP-HPLC法进行了单因素和正交试验考察提取工艺.结果表明,千斤拔有效成分的最佳提取分离工艺为:分别用石油醚、三氯甲烷脱脂,脱脂后药渣加入料液比为1∶14的醋酸乙酯100℃回流提取3h,提取2次.

In order to optimize the extraction technology of the components in Moghania philippinensis （Merr. et Rolfe） Li, RP-HPLC was adopted to examine the technology with single-factor and orthogonal experimental designs. The following conditions were identified as the best extraction technology for the compo- nents in M. philippinensis： degreasing with petroleum benzene for 3 h and with chloroform for 3 h; two backflow extractions at100℃ for 3 h after the addition of ethyl acetate with a solid： liquid ratio of 1 ： 14.