硫化氢对大鼠烧伤后炎症反应的影响

The impact of hydrogen sulfide on post-burn inflammatory response in rats

目的分析大鼠烧伤后血清中气体信号分子硫化氢(H2S)的变化及烧伤后炎性因子C-反应蛋白、白细胞介素-6及总抗氧化能力在不同H2S水平下的变化,从而探讨H2S对烧伤后炎症反应的影响。方法 78只雄性SD大鼠,随机选取72只制备30%TBSAⅢ度烫伤模型,并分为单纯烧伤组、NaHS组和PPG组,每组24只,余下6只大鼠作为非烧伤组。单纯烧伤组、NaHS组和PPG组分别在6h、12h、24h、48h时项点各取6只大鼠采集血清。采用敏感硫电极法测定血清H2S浓度,采用试剂盒分析仪分析C-反应蛋白(CRP)、白细胞介素-6及总抗氧化能力(TAOC)变化。结果与非烧伤组比较,单纯烧伤组各时相点H2S水平均降低,6h(35.18±5.46)μmol/L和12h(52.20±5.06)μmol/LH2S水平均降低较明显(P<0.05),各时相点CRP及白细胞介素-6均明显升高,TOAC降低(P<0.05);与单纯烧伤组比较,NaHS组H2S水平在伤后6h(54.77±3.12)μmol/L、12h(52.20±5.06)μmol/L和24h(50.91±3.80)μmol/L均明显升高(P<0.05);与单纯烧伤组比较,各时相点PPG组H2S水平降低,CRP及白细胞介素-6均明显升高,TOAC降低(P<0.05)。结论本实验通过了解H2S对烧伤大鼠血清白细胞介素-6、CRP及TAOC的影响,进一步表明了H2S具有抗炎及抗氧化作用,但H2S能否在严重烧伤炎症中成为新的治疗方向,尚需更多的相关研究。

Objective To explore the impact of hydrogen sulfide （n2 S） on post-burn inflammatory response in rats through analyzing the change of HRS in blood serum as well as the change of C-reaction protein （CRP）, IL-6 and total antioxidant capacity （TAOC） under different H2 S levelS. Methods Seventyeight male SD rats were randomly setected, 72 of whom were prepared as 30% TBSA full thickness burn model, and randomly divided into simple burn group, NariS group and PPG group （24 rats in each group） ; the remained 6 rats were selected as burn group, to collect the blood serum of rats at 6 h, 12 h, 24 h and 48 h respectively. The sulfur sensitive electrode method was used for the determination of serum H2S concentrations, to analyze the change of CRP, IL-6 and TAOC with relevant testing kits. Results Compared with the none burn group, the H2S level in te simple burn group decreased at each time point, especially at 6 h （35.18 ± 5.46） μmol/L and 12 h （52.20 ± 5.06） μmol/L, P 〈 0. 05; CRP, IL-6 significantly increased and TOAC decreased （ P 〈 0.05 ） at each time point ; compared with simple burn group, H2S level in NariS group significantly increased at 6 h（54.77 ± 3.12） μmol/L ）, 12 h （52.20± 5.06 ） μmol/L and 24 h （ 50.91± 3.80） μmol/L post burn, P 〈 0.05 ; compared with simple burn group, in the PPG group, CRP and IL-6 increased, H2 s level and TOAC decreased at different time point （ P 〈 0.05 ）. Conclusion Through studying the impact of H2S on IL-6, CRP, and TOAC in blood serum of burned rats the results, further indicated the anti-inflammatory and antioxidant effects of H2S, but whether H/S could be a new therapeutic direction in severe burn inflammation still needs more research.