摘要
[目的]对天麻多糖的提取工艺进行研究并测定其各组分中多糖、蛋白质和糖醛酸的含量。[方法]用正交试验对天麻多糖的提取工艺进行优选,然后采用苯酚-硫酸法、间羟基联苯法和考马斯亮兰法分别测定天麻多糖及其各组分中总糖、糖醛酸和蛋白质的含量。[结果]最佳提取工艺条件为A2B2C3,即:提取温度为70℃,提取时间为2.5 h,提取次数为3次;在此条件下,多糖提取率达13.15%。天麻多糖及其各组分中糖含量分别为65.8%、84.6%和87.3%,蛋白质含量分别为2.9%、0.28%和2.7%,糖醛酸含量分别为29.7%、26.3%和39.6%。[结论]该方法简便、快速、准确,灵敏度高,为天麻多糖生产的质量控制提供了依据。
[ Objective ] To establish a convenient and accurate method for the content determination of polysaccharides from RHIZOMA GAS- TRODIAE. [ Method] Extraction process of RHIZOMA GASTRODIAE polysaccharides was optimized by orthogonal test. The total saccharide content, uronic acid content and protein content were detected by phenol-sulfuric acid method, m-hydroxydiphenyl method, and Coomassie Brilliant method, respectively. [ Result ] The optimal extraction condition was A2B2 C3, that is 70 ℃ extraction temperature, 2.5 h extraction time, and 3 extraction times. Under this condition, the extraction rate of polysaccharides reached 13.15%. Saccharide contents in different groups of RHIZOMA GASTRODIAE polysaceharides were 65.8% , 84.6% and 87.3% ; protein contents were 2.9% , 0.28% and 2.7% ; uronic acid contents were 29.7% , 26.3% and 39.6%. [ Conclusion] This method was rapid, simple, accurate, and sensitive, and could be used for the quality control of RHIZOMA GASTRODIAE polysaccharides.
出处
《安徽农业科学》
CAS
2012年第18期9648-9650,共3页
Journal of Anhui Agricultural Sciences
基金
安徽省高等学校省级自然科学研究项目(KJ2012B209)
关键词
天麻
多糖
分光光度法
含量测定
RHIZOMA GASTRODIAE
Polysaccharides
Content determination
Spectrophotometry