摘要
目的通过检测４种常用细胞因子对白血病细胞株Ｋ５６２／Ｓ及其多药耐药细胞株Ｋ５６２／Ａ０２积蓄柔红霉素（ＤＮＲ）能力的影响，评价细胞因子在抗白血病细胞多药耐药性方面的应用前景。方法用流式细胞仪及荧光法检测重组人α－干扰素（ＩＦＮ－α）、白细胞介素２（ＩＬ－２）、肿瘤坏死因子（ＴＮＦ）、重组人粒－单集落刺激因子（ＧＭ－ＣＳＦ）对Ｋ５６２／Ｓ及Ｋ５６２／Ａ０２积蓄柔红霉素能力的影响。结果ＩＬ－２、ＴＮＦ、ＧＭ－ＣＳＦ作用后Ｋ５６２／Ｓ及Ｋ５６２／Ａ０２细胞株对柔红霉素的积蓄无改变，而ＩＦＮ－α可显著提高耐药细胞系Ｋ５６２／Ａ０２细胞内ＤＮＲ浓度，在１５０ｍｉｎ时升高了４.０１倍（Ｐ＜０.０５）。结论小剂量（５００Ｕ／ｍｌ） α－干扰素作用后，多药耐药细胞株 Ｋ５６２／Ａ０２对抗肿瘤药物ＤＮＲ的积蓄作用大大提高，提示ＩＦＮ－α具有提高耐药细胞株细胞内药物浓度、恢复其药物敏感性、逆转多药耐药性的作用。
Objective To explore measures to overcome multidrug resistance of leukemia cells. Methods The effects of 4 cytokines on intracellular daunorubicin (DNR) accumulation in K562/S and K562/A02 cells were studied by spectrofluorimetry and flow cytometric analysis. Results IFN-α(500 U/ml) could enhance the accumulation of DNR in K562/A02 cells. DNR concentration in K562/A02 was increased by 4.01 folds when cultured with IFN-α for 150 min, while the other 3 cytokines had no effect on the accumulation of DNR in K562/S and K562/A02 cells, Conclusion IFN-α(500U/ml) can markedly enhance the intracellular accumulation of DNR in K562/A02 cells.
出处
《第一军医大学学报》
CSCD
2000年第2期158-160,共3页
Journal of First Military Medical University
关键词
白血病
多药耐药性
细胞因子
药物积蓄
柔红霉素
leukemia
multidrug resistance
cytokines
accumulation effect
daunorubicin
