当归多糖的酶法提取新工艺研究

Enzymatic Extraction of Polysaccharide from Angelica

目的:研究当归多糖的酶法提取新工艺,并就提取多糖的抗肿瘤活性进行评价。方法:采用生物酶辅助提取技术进行当归多糖提取工艺研究,选用单因素实验探索酶用量、酶解温度、酶解时间及pH值对当归多糖提取率的影响,在单因素实验的基础上,采用正交试验对工艺条件进行优化,并采用MTT法评价当归多糖对hela细胞的抗肿瘤活性。结果:各因素对提取效果影响由大到小依次为:酶浓度>酶解时间>pH值>酶解温度,最佳提取工艺条件为,纤维素酶浓度:0.4mg/mL、提取温度:60℃、提取时间:4h,pH=5.0,当归多糖得率为150.34mg/g,MTT结果证实提取多糖具有一定的抗肿瘤活性。结论:该工艺操作简单,成本较低,本研究为当归多糖的深度开发提供了科学依据。

Objective： Enzymatic extraction technology of polysaecharide from Angelica and its antitumor activity were studied. Method： The effects of enzyme dosage, enzymolysis temperature, enzymolysis time, enzymolysis pH value on the extraction rate of polysaeeharide were studied by single - factor test. Then, the orthogonal experiment was performed to screen the optimum extraction technology. Antitumor activity of Angelica polysaecharide was evaluated by MTr meth- od in hela cells. Result： The factors influencing the extraction process were in the order：enzyme dosage 〉 enzymolysis time 〉 enzymolysis pH value 〉 enzymolysis temperature, and the optimum extracting technology was as follows ： enzyme dosage 0.4mg/ml, enzymolysis temperature 60℃, enzymolysis time 4h and enzymolysis pH value 5. The polysaccharide extraction rate was 150.34mg/g under these conditions. The results of MTr method confirmed that Angelica polysaccha- ride had some antitumor activity. Conclusion： The enzymatic extraction technology is simple with low cost and provides a scientific basis for the further development of Angelica polysaccharide.