IL-18基因启动子多态性与缺血性脑卒中关系

RELATIONSHIP BETWEEN INTERLEUKIN-18 PROMOTER POLYMORPHISM AND ISCHEMIC STROKE

目的研究白细胞介素-18(IL-18)基因启动子-607C/A、-137G/C位点基因多态性与缺血性脑卒中的关系。方法采用病例对照研究方法,选择193例缺血性脑卒中病人(病例组)和性别、年龄等指标与之完全匹配的健康人120例为研究对象。采用聚合酶链反应技术检测IL-18基因启动子-607C/A、-137G/C基因多态性。采用Logistic回归分析方法分析上述位点基因多态性与缺血性脑卒中的相关性。结果病例组-607C/A位点CC型频率明显高于对照组(χ2=10.248,P<0.01),-607C/A位点非携带A等位基因者患缺血性脑卒中的风险约是携带A等位基因者的2.6倍(OR=2.603,P<0.05)。两组-137G/C位点基因型及等位基因分布差异均无统计学意义。结论 -607C/A位点基因多态性与缺血性脑卒中的发病风险存在相关性,A等位基因可能对缺血性脑卒中的发病起保护作用,而C等位基因携带者患缺血性脑卒中的危险性增大;-137G/C位点基因多态性与缺血性脑卒中风险可能不存在相关性。

Objective To study the relationship between polymorhpism of IL-18 promoter -607C/A and -137G/C and ischemic stroke （IS）. Methods A case-control study was done in 193 IS patients and 120 healthy controls who matched the patients＇ sex and age. By using polymerase chain reaction （PCR） technique, the gene polymorphism at -607C/A and -137G/C was detected. The association of the gene polymorphism with IS was analyzed by logistic regression. Results In the IS patients, at -607C/A gene site, the frequency of genotype CC was higher than that in the control （X2= 10. 248, P〈0.01）, and -607C allele was associated with a higher risk of IS （OR =2. 603, P〈0.05）. No significant difference was found between the two groups in terms of distribution of genotypie frequency and allelic frequency in -137G/C gene site. Conclusion The IL-18 promoter gene polymorphism at position -607 is associated with IS, A allele may be a protection against this condition, and the C allele carries are at high risk of IS. It is probably that gene polymorphism at position -137 is not associated with this disease.