早产儿血清S100B蛋白与脑损伤的关系

Relationship between serum S100B protein level and brain damage in preterm infants

目的探讨血清S100B蛋白在早产儿脑损伤中的变化及意义。方法 47名早产儿纳入本研究,根据影像学MRI和头颅B超检查结果分为无脑损伤组(20例)、非脑白质损伤组(14例)和脑白质损伤组(13例)。留取生后﹤24 h、72 h及7 d的血清样本,采用酶联免疫吸附测定法(ELISA)双抗体夹心法测定S100B蛋白水平。结果脑白质损伤组和非脑白质损伤组出生后24 h、72 h及7 d血清S100B蛋白水平均明显高于无脑损伤组(P<0.05),且脑白质损伤组血清S100B蛋白水平在各监测时间点明显高于非脑白质损伤组(P<0.05)。结论脑损伤早产儿生后7 d内血清S100B蛋白水平增高,提示血清S100B蛋白可以作为一种脑损伤的早期敏感标志物,对于临床早产儿脑损伤特别是脑白质损伤的诊断更有意义。

Objective To study changes of serum S100B protein level in preterm infants with brain damage and its role. Methods Forty-seven preterm infants were classified into 3 groups based on the results of brain ultrasound and MRI ： brain white matter damage （WMD ; n = 13）, brain but not white matter damage （ non-WMD ; n = 14 ） and control （no brain damage; n ： 20）. Blood samples were collected within 24 hrs, 72 hrs and 7 days after birth. S100B protein level was measured using ELISA. Results Serum levels of S100B in the WMD and non-WMD groups were significantly higher than in the control group within 24 hours, 72 hours and 7 days after birth （P 〈 0.05 ）. More increased serum S100B levels were observed in the WMD group compared with the non-WMD group （P 〈 0.05）. Conclusions Serum S100B protein level increases in preterm infants with brain damage within 7 days after birth, suggesting that it may be used as an early sensitive marker for the diagnosis of brain damage, especially WMD.