反相高效液相色谱法测定甲磺酸酚妥拉明速崩片的含量

Determination of Phentolamine Mesylate in Rapidly Disintegrating Tablet by RP-HPLC

目的： 建立１ 种用高效液相色谱检测甲磺酸酚妥拉明速崩片含量的方法。方法： 用ＹＷＧ－ Ｃ１８ 柱为固定相。０－０１ ｍｏｌ·Ｌ－ １ 磷酸［含０－１３％ （ｖ／ｖ） 三乙胺］ － 乙腈（３０∶７０） 为流动相， ＵＶ 检测波长２７８ ｎｍ 。结果：该方法甲磺酸酚妥拉明回收率为１００－９ ％ ， ＲＳＤ 为０－５ ％ （ｎ ＝６） 。结论： 该法准确可靠， 专一性强， 能满足制剂质量标准的要求。

Objective:A RP-HPLC method has been studied to determine the contents of rapidly disintegrating tablet of phentolamine mesylate. Method:In the method μ-Bondapak ODS as the stationary phase and 0 01 mol·L -1 H 3PO 4(including 0 13%( v/v )triethylamine)-acetonitrile (30∶70)as the mobile phase were adopted, the detection wavelength of 278 nm was specified and naphthalene was used as internal standard. The flow rate was 1 0 mL·min -1 .Results:There is a good linear relationship within the range of 5 0～100 0 μg·mL -1 ( r=0 9999) and the average recovery was 100 9%( n=6,RSD=0 5%). Conclusion:The method is simple, rapid and reliable.