尾静脉移植人脂肪干细胞在脑缺血大鼠脑内的存活

Survival of human adipose-derived mesenchymal stem cells transplanted via the tail vein in rat brain with cerebral ischemia

背景:间充质干细胞移植可显著改善缺血性脑血管病神经功能。目的:观察尾静脉途径移植人脂肪源间充质干细胞对局灶性脑缺血大鼠神经功能的影响。方法:制作局灶性脑缺血大鼠模型,随机分为实验组和对照组,实验组在造模后3d尾静脉注射5×106脂肪源间充质干细胞,对照组不进行细胞移植。结果与结论:两组大鼠神经功能缺损行为学评分随着细胞移植后时间延长均逐渐降低。移植后第14,21天,实验组大鼠行为学评分较对照组显著降低(P<0.05),且实验组改善程度明显优于对照组(P<0.01);移植的人脂肪源间充质干细胞在局灶性脑缺血大鼠血管周边和缺血周边区聚集并存活。说明尾静脉移植的脂肪源间充质干细胞可在宿主脑内存活,并改善神经功能。

BACKGROUND： Mesenchymal stem cells transplantation can improve the neurological function of ischemic cerebrovascular disease. OBJECTIVE： To explore the effect of human adipose-derived mesenchymal stem cells transplanted via the tail vein on functional recovery in rats with focal cerebral ischemia. METHODS： Focal cerebral ischemia rat models were established, and then rat models were divided into experimental group and control group. In the experimental group, 5x106 adipose-derived mesenchymal stem cells were injected into rat models via the tail vein, and the rat models in the control group were treated without cell transplantation. RESULTS AND CONCLUSION： Behavioral score of neurological defects in two groups was gradually reduced with the time prolonged after cell transplantation. At 14 and 21 days after transplantation, the behavioral score of rats in the experimental group was significantly lower than that in the control group （P 〈 0.05） and the improvement of experimenta group was significantly better than that of the control group （P 〈 0.01）; transplanted adipose-derived mesenchymal stem cells were gathered and survived surrounding the blood vesselss and periischemic areas in rats with focal cerebral ischemia. Adipose-derived mesenchymal stem cells transplanted via the tail vein can survive in the host brain and improve neurological function.