庄河大骨鸡ESR基因多态性及其与产蛋性能关联的分析研究

Correlation Analysis between Polymorphism of ESR Gene and Laying Performance in Zhuanghe Dagu Chicken

为了探究庄河大骨鸡ESR基因的多态性与产蛋性能相关联的分子遗传标记,为庄河大骨鸡的选育奠定理论基础。以庄河大骨鸡为试验材料,利用PCR-SSCP标记技术分析了ESR基因的5'侧翼序列。结果表明:ESR基因在庄河大骨鸡中存在3种基因型,即:CC型、CD型和DD型,3种基因型的频率分别为:0.245、0.335、0.42,C基因和D基因频率为0.4125和0.5875;CC型的产蛋数、蛋料比显著高于其他2种基因型,差异显著(P<0.05),开产周龄、体重、产蛋高峰期和蛋重3种基因型间差异不显著(P>0.05)。ESR基因的CC型对鸡的产蛋性能有显著影响,但是否能作为庄河大骨鸡选育的重要理论依据还有待进一步研究。

The aim of the present study was to investigate the correlation between polymorphismm of flanking sequence of ESR gene and laying performance of Zhuanghe Dagu Chickens, providing the theoretical foundation for selection of Zhuanghe Dagu Chickens. Zhuanghe Dagu Chickens were used to analyze 5＇ flanking sequence of ESR gene by PCR-SSCP method. The results showed that ESR was consisted of three genotypes （CC, CD and DD） and frequencies of the three genotypes were 0.245, 0.335 and 0.42 respectively. Frequencies of C gene and D gene were 0.4125 and 0.5875 respectively. Egg number and ratio of feed and egg of chickens with CC genotype were significantly higher than those of CD and DD genotype （P〈0.05）. There were no significantly differences in age and body weigh at first laying, laying peak and egg weight among the three genotypes （P〉0.05）. In conclusion, CC genotype of ESR could significantly affect laying performance of Zhuanghe Dagu Chickens, however, it need further study to confirm that ESR gene could be used as the important theoretical basis for selection of Zhuanghe Dagu Chickens.