结直肠息肉水通道蛋白3、4的表达

Expression of aquaporin 3 and 4 proteins in colorectal polyps

目的:观察水通道蛋白(aquaporin,AQP)3、4蛋白在结直肠息肉中的表达.方法:选择结直肠息肉患者103例,肠镜下收集115个息肉组织样本和10例正常人结直肠黏膜组织样本,采用免疫组织化学方法检测AQP3、4的蛋白表达.结果:AQP3、4蛋白在人结直肠黏膜上皮细胞呈阳性表达,均表达在细胞的基底部与侧面.AQP3、4蛋白在炎性息肉中的表达均明显高于正常组,差异有显著性意义(P<0.01,P<0.05);增生性息肉AQP3蛋白表达低于正常组,差异有显著性意义(P<0.01);腺瘤AQP4蛋白表达低于正常组,差异有显著性意义(P<0.01).AQP3与AQP4在结直肠息肉及正常结直肠黏膜中的表达呈正相关(r=0.61,P=0.000).结论:AQP3、4蛋白在结直肠息肉中存在异常表达,二者之间呈正相关.

AIM： To investigate the expression of aquaporin 3 and 4（AQP3,4） proteins in colorectal polyps.METHODS： One hundred and three patients with colorectal polyp and 10 healthy volunteers were selected,and 115 polyp tissue samples and 10 normal bowel mucosal tissue samples were collected from these subjects by colonoscopy.The protein expression of AQP3 and 4 was detected by immunohistochemistry.RESULTS： The expression of AQP3 and 4 proteins was detected in colorectal epithelial cells that were located in the basolateral membrane.The protein expression of AQP3 and 4 in the inflammatory polyps group was significantly higher than that in the normal group（P 〈 0.01,P 〈 0.05）.The protein expression of AQP3 was significantly lower in hyperplastic polyps and adenomas than in normal bowel mucosal tissue（both P 〈 0.01）.There was a positive correlation between AQP3 and AQP4 expression in colorectal polyps and normal bowel mucosa（r = 0.61,P = 0.000）.CONCLUSION： AQP3 and 4 proteins are abnormally expressed in colorectal polyps and there is a positive correlation between AQP3 and AQP4 expression.