期刊文献+

Proteomic profiling of the hemolymph at the fifth instar of the silkworm Bombyx mori 被引量:1

Proteomic profiling of the hemolymph at the fifth instar of the silkworm Bombyx mori
原文传递
导出
摘要 Two-dimensional gel electrophoresis (2-DE) followed by matrix-assisted laser desorption ionization- time-of-flight/time-of-flight mass spectrometry (MS) analysis were used to charaterize the hemolymph proteomic profiles of the silkworm, Bombyx mori. At days 4 (V4) and 5 (VS) of the fifth (final) instar, when the larvae were at the fast-growing stage, we found dramatic changes in spots representing proteins having an approximate molecular weight (MW) of 30 kDa. Of these spots, four 30K proteins were highly up- regulated, implying a close association with the growth and development ofB. mori larvae. To understand the molecular basis and underlying mechanisms involved in development and metamorphosis, the proteome of whole hemolymph at V5 was analyzed using shotgun liquid chromatography tandem mass spectrometry with an LTQ-Orbitrap. A total of 108 proteins were identified without any false discovery hits. These proteins were involved in a variety of cellular functions, including metabolism, development, nutrient transport and reserve, and defense response. Gene ontology analysis showed that 3.4% of these proteins had nutrient reservoir activities and 5.7% were involved in the response to stimulus. Pathway analysis revealed that 22 proteins with common targets were involved in various cellular processes such as immunity, differentiation, proliferation and metamorphosis. These results suggested that some key factors such as the 30K proteins in hemolymph play important roles in B. mori growth and development. Moreover, the multiple functions of hemolymph may be operated by a complex biological network. Two-dimensional gel electrophoresis (2-DE) followed by matrix-assisted laser desorption ionization- time-of-flight/time-of-flight mass spectrometry (MS) analysis were used to charaterize the hemolymph proteomic profiles of the silkworm, Bombyx mori. At days 4 (V4) and 5 (VS) of the fifth (final) instar, when the larvae were at the fast-growing stage, we found dramatic changes in spots representing proteins having an approximate molecular weight (MW) of 30 kDa. Of these spots, four 30K proteins were highly up- regulated, implying a close association with the growth and development ofB. mori larvae. To understand the molecular basis and underlying mechanisms involved in development and metamorphosis, the proteome of whole hemolymph at V5 was analyzed using shotgun liquid chromatography tandem mass spectrometry with an LTQ-Orbitrap. A total of 108 proteins were identified without any false discovery hits. These proteins were involved in a variety of cellular functions, including metabolism, development, nutrient transport and reserve, and defense response. Gene ontology analysis showed that 3.4% of these proteins had nutrient reservoir activities and 5.7% were involved in the response to stimulus. Pathway analysis revealed that 22 proteins with common targets were involved in various cellular processes such as immunity, differentiation, proliferation and metamorphosis. These results suggested that some key factors such as the 30K proteins in hemolymph play important roles in B. mori growth and development. Moreover, the multiple functions of hemolymph may be operated by a complex biological network.
出处 《Insect Science》 CAS CSCD 2012年第4期441-454,共14页 昆虫科学(英文版)
关键词 Bombyx mori gene ontology HEMOLYMPH LTQ-Orbitrap pathway proteomics Bombyx mori, gene ontology, hemolymph, LTQ-Orbitrap, pathway,proteomics
  • 相关文献

参考文献35

  • 1Bogaerts, A., Baggerman, G., Vierstraete, E., Schools, L. and Verleyen, P. (2009) The hemolymph proteome of the honey- bee: Gel-based or gel-free? Proteomics, 9, 3201-3208.
  • 2Brennan, C.A. and Anderson, K.V. (2004) Drosophila: The genetics of innate immune recognition and re- sponse. Annual Review of Immunology, 22, 457- 483.
  • 3Dammai, V., Adryan, B., Lavenburg, K.R. and Hsu, T. (2003) Drosophila awd, the homolog of human nm23, regulates FGF receptor levels and functions synergistically with shi/dynamin during tracheal development. Genes & Development, 17, 2812-2824.
  • 4Elias, J.E. and Gygi, S.R (2007) Target-decoy search strategy for increased confidence in large-scale protein identifications by mass spectrometry. Nature Methods, 4, 207-214.
  • 5Gene Ontology Consortium (2008) The gene ontology project in 2008. Nucleic Acids Research, 36 (Database issue), D440-D444.
  • 6Haunerland, N.H. (1996) Insect storage proteins: gene families and receptors. Insect Biochemistry and Molecular Biology, 26, 755-765.
  • 7Hou, Y., Zou, Y., Wang, E, Gong, J., Zhong, X., Xia, Q. and Zhao, E (2010) Comparative analysis of proteome maps of silkworm hemolymph during different developmental stages. Proteome Science, 8, 45.
  • 8Iketani, M., Nishimura, H., Akayama, K., Yamano, Y. and Morishima, I. (1999) Minimum structure of peptidoglycan required for induction of antibacterial protein synthesis in the silkworm, Bombyx mori. Insect Biochemistry and Molecular Biology, 29, 19-24.
  • 9International Silkworm Genome Consortium (2008) The genome of a lepidopteran model insect, the silkworm Bom- byx mori. Insect Biochemistry and Molecular Biology, 38, 103(~1045.
  • 10Izumi, S., Fujie, J., Yamada, S. and Tomino, S. (1981) Molec- ular properties and biosynthesis of major plasma proteins in Bombyx mori. Biochimica et Biophysica Acta (BBA) Protein Structure, 670, 222-229.

同被引文献2

引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部