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一株产L-赖氨酸菌株发酵培养基的响应面优化 被引量:3

Optimization of the Fermentation Medium of a L-lysine-producing Strain by Response Surface Methodology
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摘要 本试验优化了一株黄色短杆菌HXL109的发酵培养基以提高L-赖氨酸的产量。在研究葡萄糖、硫酸铵、豆饼水解液、KH2PO4.3H2O、MgSO4.7H2O、FeSO4.7H2O、MnSO4.H2O七个单因素实验的基础上,用Design Expert软件的Box-Behnken Design(BBD)建立响应面模型。结果表明:HXL109最佳产酸条件为:葡萄糖89.48 g/L,豆饼水解液30.77 g/L,硫酸铵20.89 g/L,KH2PO4.3H2O 4.5g/L。在此条件下L-赖氨酸的产量为142.65 g/L,与预测值(143.67 g/L)吻合度较高。通过发酵对比实验可见,用响应面分析法对该L-赖氨酸产生菌发酵培养基进行优化,可获得最佳的工艺条件。 The fermentation medium of a L-lysine producing swain Brevibacterium flavum HXL109 to improve the L-lysine production was optimizd. With the software Design Expert, a response surface model was established using the Box-Behnken design(BBD). The effects of glucose, (NH4)2SO4, soybean cake hydrolysate, KH2PO4·3H2O, MgSO4·7H2O, FeSO4·7H2O and MnSO4·H2O on L-lysine yield were analyzed. The results showed that the optimum conditions for HXL109 to produce L-lysine were as follows: glucose 89.48g/L, soybean cake hydrolysate 30.77g/L, (NH4)2SO4 20.89g/L and KH2PO4·3H2O 4.5g/L. Under these conditions, the result of confirmatory experiment (142.65g/L) was in good agreement with the predicted value (143.67g/L). A comparison study showed that the established regression model had good effectiveness in optimizing the fermentation medium of a L-lysine producing sWain.
出处 《现代食品科技》 EI CAS 北大核心 2012年第8期1049-1053,1087,共6页 Modern Food Science and Technology
关键词 L-赖氨酸 响应面 对比实验 L-lysine RSM comparing experiment
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