复方土茯苓颗粒对高尿酸血症肾病模型小鼠肾功能及IL-1β,IL-6表达的影响

Effects of Fufang Tufuling Granules on IL-1β, IL-6 in the Mouse Uratic Renal Injury

目的:观察复方土茯苓颗粒对高尿酸血症肾病模型小鼠血尿酸、肾功能的影响及肾脏组织中白细胞介素1(IL-1β)、白细胞介素6(IL-6)表达的影响。方法:采用腺嘌呤(100 mg.kg-1)联合97%氧嗪酸钾(1 g.kg-1)ig法建立小鼠高尿酸血症肾病动物模型,设立正常对照组、造模组、阳性药物组苯溴马隆组20 mg.kg-1、复方土茯苓颗粒高、中、低剂量组(6,3,1.5 g.kg-1)。实验第21天小鼠眼球采血后处死,检测血清中尿酸、肌酐、尿素氮及肾组织中IL-1β,IL-6水平。结果:复方土茯苓颗粒高、中、低剂量组尿酸(110.22±28.07),(128.10±54.79)(144.10±51.73)μmol.L-1;肌酐(39.11±8.55),(60.90±21.29),(87.50±16.18)μmol.L-1;尿素氮(25.15±3.17),(29.65±2.17),(35.01±3.63)mmol.L-1;IL-1β(3.13±0.53),(4.26±1.44),(5.10±1.86)μg.L-1;IL-6(14.90±4.93),(22.87±6.71),(35.83±22.19)μg.L-1;与模型组血尿酸(259.22±31.05)μmol.L-1、肌酐(103.00±22.34)μmol.L-1,尿素氮(50.76±9.93)mmol.L-1,IL-1β(10.74±4.0)μg.L-1,IL-6(62.87±15.42)μg.L-1相比明显降低(P<0.05),复方土茯苓颗粒高、中剂量组与苯溴马隆组相比,组间比较无统计学差异。结论:复方土茯苓颗粒能够有效降低高尿酸血症肾病模型小鼠尿酸、肌酐、尿素氮水平,保护尿酸造成的肾脏损伤,可能机制为降低IL-1β,IL-6等炎症因子的表达。

Objective： To observe the effect of Fufang Tufuling granules on serum level of uric acid （UA）, Urea nitrogen （BUN）, creatinine （Cr）, and the level of interleukin 1β（IL-1β）, interleukin 6 （IL-6） in mouse kidriey homogenate. Method： Animal model was made by Adenine （100 mg·kg^-1） and uricase （1 g·kg^-1）. The animals were divided into normal group, model group, positive drug Benzbromarone 20 mg·kg^-1 group, Fufang Tufuling granules high dose group, Fufang Tufuling granules middle dose group, Fufang Tufuling granules low dose group （6, 3, 1.5 g·kg^-1 ）. At the 21th day, blood was collected, then the serum level of UA, BUN, CR, and the IL-1β, IL-6 in Kidney homogenate were measured. Result： The serum level of UA [ （110.22±28.07）, （128.10±54.79）, （144.10±51.73） μg·L^-1], BUN [ （25.15±3.17, （29.65± 2.17）, （35.01±3.63） mmol·L^-1], Cr [ （39.11 ±8.55） , （60.90±21.29） , （87.50 ±16. 18）μg·L^-1], and the level oflL-1β [ （3.13±0.53） , （4.26±1.44） , （5.10±1.86）μg·L^-1], IL-6 [ （14.90±4.93）, （22.87±6.71 ）, （35.83±22. 19） μg·L^-1 in kidney homogenate in Fufang Tufuling granules high, middle and low dose group was lower than those in the model group （P 〈 0.05）. The effects of high and medium dose group were similar to Benzbromarone group. Conclusion： The Fufang Tufuling granules can prevent and cure experimental hyperuricemia and hyperuricemic renal impairment. The mechanism might be related to inhibiting the expressions of IL-1β and IL-6.