犀黄丸对大肠癌LoVo细胞基质金属蛋白酶2,9蛋白表达的影响

Effect of Xihuang pills on protein expressions of MMP-2 and MMP-9 in human colorectal carcinoma LoVo cell

[目的]研究犀黄丸含药血清对大肠癌LoVo细胞中基质金属蛋白酶-2,9(MMP-2,MMP-9)表达的影响及意义。[方法]应用酶联免疫吸附试验(ELISA)方法,检测犀黄丸含药血清作用下LoVo细胞培养上清液中MMP-2、MMP-9的蛋白表达水平。[结果]在犀黄丸含药血清的干预下,LoVo细胞中MMP-2、MMP-9的蛋白分泌水平与空白对照组比较明显下调,差异具有统计学意义(P<0.05)。[结论]犀黄丸可能通过抑制MMP-2和MMP-9的蛋白表达,达到抑制大肠癌转移的作用。

[Objective] To investigate the effect of Xihuang pills（medicine-contained serum） on the protein expressions of matrix metallo- proteinase-2（MMP-2） and matrix metailoproteinase-9（MMP-9） in human colorectal carcinoma LoVo cell. [Methods] The enzyme-linked immunoassay （ELISA） was used to determine the expression of MMP-2 and MMP-9 in the culture solution of LoVo cell under the action of Xihuang pills. [Results] The expression of MMP-2 and MMP-9 in medication groups was significantly lower than those of control group （P〈0.05）. [Conclusion] Xihuang pill has a potent effect of reducing the expressions of MMP-2 and MMP-9. This mechanism may play an important role in the suppression of colorectal carcinoma metastasis.