胶原蛋白海绵复合bFGF促进兔胫骨外露创面愈合的实验研究

Basic Fibroblast Growth Factor and Collagen Sponge Improve Rabbits Skin Wound Healing with Exposed Tibia

目的研究胶原蛋白海绵复合bFGF对骨外露创面愈合的影响。方法 30只日本大耳白兔,随机分为A、B、C三组,每组10只。于胫骨上段前内侧作一1cm×1.5cm创面,暴露胫骨。A组创面喷洒bFGF后,胶原蛋白海绵覆盖,纱布包扎。B组覆盖胶原蛋白海绵扎。C组喷洒生理盐水。术后观察各组创面完全愈合的时间,测量并计算术后各时期创面收缩率。创面愈合后,取材,HE染色,显微镜下观察组织结构。结果大体观察:A、B、C组创面愈合时间分别为(25.12±1.46)d、(30.50±1.69)d、(33.38±1.77)d。A组较B、C组愈合时间提前,差异有统计学意义(P<0.01)。组织学观察:A组创面愈合质量优于B、C组。结论胶原蛋白海绵复合bFGF对兔胫骨外露创面愈合具有促进作用,提高创面愈合质量,减少瘢痕组织。

Objective To study the effect of bFGF and collagen sponge on rabbits skin wound healing with exposed tibia. Methods 30 Japanese white rabbits, were divided into A, B,C three groups according to the different treatment methods, n = 10. A long 1.5cm and wide lcm skin wound was made in each rabbit＇s front anteromedial tibia. Experimental group A of wounds were treated with bFGF, covered collagen sponge and packed with gauze. Group B of wounds were covered with collagen sponge and packed with gauze.Group C of wounds were treated with the same dose of 0.9% saline solution and packed with gauze. Each wound was dressed one time every three days ,until the skin wound was healed completely.when wound was dressed ,we took photographs by a digital camera, measured the size of skin wound and calculated the rate of wound contraction. When skin wound was healed, we cut wound tissue and make specimens. Each specimen was stained in HE coloring. We observed and compared microscope structure under light microscope. Results The average healing time of group A wounds was 25.12 ±1.46 days, It was 30.50±1.69 days in group B. It was 33.38 ±1.77 days in group C. The average healing time of experimental group A was shorter than that of control group B and group C, and the results were significantly different （P〈 0.01）. Light microscope observation showed that the quality of the experimental group healed wound was much better than the control group. Conclusion We conclude that bFGF and collagen sponge significantly promote rabbits skin wound healing with exposed tibia and improve the quality of the healed wound.