赶黄草总黄酮超声提取工艺的响应面法优化及其体外抗氧化活性分析

Optimization of Ultrasonic-assisted Extraction Technology for Total Flavonoids from Penthorum chinense by Response Surface Methodology and Evaluation Its in vitro Antioxidant Activity

目的:优化赶黄草总黄酮的超声提取工艺并分析其体外抗氧化活性。方法:以总黄酮得率为指标,在单因素试验基础上,采用响应面设计法对影响总黄酮得率的乙醇体积分数、超声时间及料液比进行优化,同时考察其体外抗氧化活性。结果:赶黄草总黄酮超声提取的最佳工艺条件为26倍量60%乙醇于50℃下超声提取20 min。在此条件下,总黄酮得率4.14%,试验结果与模型预测值相符。体外抗氧化活性研究表明,赶黄草总黄酮具有较强的还原力和总抗氧化能力,同时具有较强的清除羟基自由基和DPPH自由基能力。结论:该优选工艺方便、快捷、高效;赶黄草总黄酮具有较强的抗氧化活性,值得深入研究。

Objective： To optimize ultrasonic-assisted extraction technology of total flavonoids from Penthorum chinense and evaluate its in vitro antioxidant activity. Method： With,yield of total flavonoids as index, the concentration of ethanol, extraction time, liquid-material ratio were optimized by response surface methodology based on single-factor tests, and its in vitro antioxidant activity were evaluated by multi-test systems. Result： Optimal extraction technology conditions were： extracted 20 min with 26 times the amount of 60% ethanol at 50 ~C. Under these conditions, yield of total flavonoids reached 4.14% , which was agreed with model predictions. In vitro antioxidant activities results showed that total flavonoids from P. chinense had good antioxidant activities of scavenging DPPH free radical and hydroxyl free radical, but also had strong reducing power and total antioxidant capability. Conclusion： Optimized ultrasonic-assisted extraction technology was simple, rapid and efficient. Total flavonoids from P. chinense had good antioxidant activity which was worth of further study.