单色光对肉鸡体质量和小肠免疫应激相关因子IL-6与TNF-α表达的影响

Effect of Monochromatic Light on Body Weight and Expression of IL-6 and TNF-α in Small Intestine of Broilers

本试验旨在通过检测在单色光下饲养的肉鸡的体质量和空肠中细胞因子白细胞介素-6(IL-6)与肿瘤坏死因子(TNF-α)表达量的改变,探讨单色光对肉鸡生长发育和肠道免疫应激水平的影响。选用256只刚出壳的雄性AA肉鸡,随机分成4组,分别在蓝(480nm)、绿(560nm)、红(660nm)、白(400～700nm)4种光色下饲养。各组饲养管理制度相同,光照的强度为15lx,光照时间为23h。肉鸡42日龄时,称重后取空肠,用ELISA测定IL-6和TNF-α的表达量。试验结果显示与白光组肉鸡相比,蓝光组肉鸡的体质量高15.2%,而空肠IL-6和TNF-α的表达量分别降低了46.8%和33.4%(P<0.05),绿光组、红光组、白光组3组间差异均不显著(P>0.05)。结果表明蓝光能促进肉鸡生长和缓解光刺激诱发的小肠免疫应激。

The aim of this study was to discover the effect of monochromatic light on the body weight and expression of IL-6 and TNF-α in the jejunum in broilers, which are two indicators of the stress degree, and define the role that monochromatic light plays towards the growth and im- mune stress procedure of intestine. 256 AA male broilers were randomly divided into 4 groups and exposed to blue light （480 nm, BL）, green light （560 nm, GL）, red light （660 nm, GL） and white light （400-700 nm, WL） at 1 day old, respectively. All light sources were equalized on the light illumination of 15 lx and schedule was L ： D = 23 h ： 1 h. At 42 days old, the broilers were weighed, and jejunums were removed to detect the expression of IL-6 and TNF-α by ELISA. The results were as follows： 1） The body weigh of the BL group was 15.2% larger that of the WL group. 2） The expression of IL-6 and TNF-α were significantly decreased by 46.8% and 33.4% in the BL group than that of WL group, respectively （P〈0.05）. However, no sig- nificant differences were observed between GL, RL and WL groups. These results indicated that the blue light can improve the growth in broilers and attenuated light-induced immune stress in the small intestine.